DNA Polymerase I (10 U/μL)

Name: DNA Polymerase I (10 U/μL)
SKU: EP0042
Price: 405.00 USD

Thermo Scientific DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonucleaseRead more

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Catalog Number	Quantity
EP0042	2500 U
EP0041	500 U

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Catalog number EP0042

Price (USD)

405.00

Each

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Quantity:

2500 U

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Price (USD)

405.00

Each

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Thermo Scientific DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.

Highlights

• Incorporates modified nucleotides (e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
• Active in multiple buffers, including restriction enzyme, PCR, and RT buffers

Applications

• DNA labeling by nick-translation in conjunction with DNase
• Second-strand synthesis of cDNA in conjunction with RNase H

For Research Use Only. Not for use in diagnostic procedures.

Specifications

DescriptionDNA Polymerase I

PolymeraseDNA Polymerase I

Quantity2500 U

Concentration10 U/μL

Unit SizeEach

Frequently asked questions (FAQs)

What are the differences between Thermo Scientific DNA Polymerase I, Klenow Fragment, and Klenow Fragment (exo-)?

Thermo Scientific DNA Polymerase I possesses 5′?3′ DNA synthesis activity, 3′?5′ exonuclease (proofreading) activity, and 5′?3′ exonuclease activity. Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5′?3′ polymerase activity and 3′?5′ exonuclease (proofreading) activity, but lacks 5′?3′ exonuclease activity. Klenow Fragment, exo-, is also the large fragment of DNA polymerase I. It exhibits 5′?3′ polymerase activity, but lacks the 3′?5′ and 5′?3′ exonuclease activities.