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Thermo Scientific™
RNase T1 (1000 U/μL)
Thermo Scientific RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues. It cleaves the phosphodiester bondRead more
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Catalog Number
Quantity
EN0542
500,000 U
EN0541
100,000 U
2 Options
Catalog number EN0542
Price (USD)
349.00
Each
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Quantity:
500,000 U
Request bulk or custom format
Price (USD)
349.00
Each
Add to cart
Thermo Scientific RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues. It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.
RNase T1 does not require metal ions for activity.
Applications
• Removal of RNA from DNA preparations • RNA sequencing • Ribonuclease protection assays. Used in conjunction with RNase A • Removal of RNA from recombinant protein preparations • Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a 'G-less cassette'
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1000 U/μL
EnzymeRNase
Quantity500,000 U
Product TypeRNase T1
Unit SizeEach
Frequently asked questions (FAQs)
How much Thermo Scientific RNase T1 should I use for complete digestion of RNA?
We would recommend 0.2 µL RNase T1 per µg of RNA in 50 mM Tris-HCl (pH 7.5) and 1 mM EDTA. Incubate at 37 degrees C for 30 min.