DH10B Competent Cells

Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently. These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.

• High transformation efficiency: >1x10⁹ cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Allows for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
DH10B Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning both of prokaryotic and eukaryotic origin
• Cloning of large plasmids and BACs
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site directed mutagenesis

Genotype
F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(Str^R) nupG

Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542).