NuPAGE™ Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0 mm, 2D-well
NuPAGE™ Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0 mm, 2D-well
Invitrogen™

NuPAGE™ Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0 mm, 2D-well

NuPAGE™ Novex™ 3–8% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE™ Tris-Acetate SDS RunningRead more
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Catalog number EA0376BOX
Price (USD)
189.00
Each
Add to cart
Price (USD)
189.00
Each
Add to cart
NuPAGE™ Novex™ 3–8% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE™ Tris-Acetate SDS Running Buffer. NuPAGE™ Novex™ Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system.

Formulation
NuPAGE™ Novex™ Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS.

Recommended buffers
Run NuPAGE™ Novex™ Tris-Acetate gels with NuPAGE™ Tris-Acetate SDS Running Buffer. To ensure good sample reduction and band resolution, use NuPAGE™ Sample Preparation Reagents with these gels. The use of NuPAGE™ Transfer Buffer provides optimal conditions for transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis. For native running conditions, the Tris-Glycine Native running and sample buffers should be used with NuPAGE™ Novex™ Tris-Acetate gels.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage3 to 8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeTris-Acetate
Product TypeGradient Page
Separation ofProtein
Separation Range36 to 500 kDa
Separation TypeDenaturing, Native
Shelf Life6 Months
Wells2D-well
Mode of SeparationMolecular Weight
Product LineNovex™, NuPAGE™
Quantity10 gels (1 box)
Shipping ConditionWet Ice
System TypeZOOM™
Thickness1.0 mm
Well Design2D Well
Length (Metric)8 cm
Length (Metric) Gel8 cm
Width (Metric)8 cm
Width (Metric) Gel8 cm
Unit SizeEach
Contents & Storage
One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 6 months.

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What does it mean when bands appear to be getting narrower (or "funneling") as they progress down a protein gel?

There may be too much beta-mercaptoethanol (BME), sample buffer salts, or dithiothreitol (DTT) in your samples. If the proteins are over-reduced, they can be negatively charged and actually repel each other across the lanes causing the bands to get narrower as they progress down the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0 mm, 2D-well FAQs