EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays
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Invitrogen™

EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

The EnzChek™ Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid,Read more
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Catalog number E6639
Price (USD)
516.00
Each
Add to cart
Price (USD)
516.00
Each
Add to cart
The EnzChek™ Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity.

See our complete line of Fluorescence Microplate assays.

• Generalized mechanism allows for measurement of a variety of proteases
• Can be used to continuously measure protease kinetics
• Excitation/emission similar to Texas Red™ dye (589/617 nm)

This EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY™ TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY™ TR-X dye has excitation and emission spectra similar to those of the Texas Red™ fluorophore.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence Intensity
For Use With (Application)Protease Assay
For Use With (Equipment)Fluorescence Microplate Reader
Product TypeProtease Assay
Product LineEnzChek™
Quantity1 kit
Shipping ConditionRoom Temperature
Substrate PropertiesProtein-Based Substrate
Substrate TypeProtease Substrate
Target EnzymeProteases
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.

What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long is the reconstituted BODIPY substrate in the EnzChek Protease Assay Kit, red fluorescence, 100-1000 assays (Cat. No. E6639) stable?

Reconstituted BODIPY casein substrates may be stored at 4 degrees C for 2-4 weeks. We recommend the addition of sodium azide at a final concentration of 2 mM to act as a preservative. If longer storage is required, freeze at -20 degrees C. Protect from light and avoid repeated freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.