ER-Tracker™ Red (BODIPY™ TR Glibenclamide), for live-cell imaging
ER-Tracker™ Red (BODIPY™ TR Glibenclamide), for live-cell imaging
Invitrogen™

ER-Tracker™ Red (BODIPY™ TR Glibenclamide), for live-cell imaging

ER-Tracker Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained usingRead more
Have Questions?
Catalog number E34250
Price (USD)
494.00
Each
Add to cart
Price (USD)
494.00
Each
Add to cart
ER-Tracker Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K+ channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed
Excitation Wavelength Range587⁄615
For Use With (Equipment)Fluorescence Microscope
Quantity100 μg
Detection MethodFluorescence
Product LineBODIPY™, ER-Tracker™
Shipping ConditionRoom Temperature
Label TypeBODIPY Dyes
Product TypeDye
SubCellular LocalizationEndoplasmic Reticulum
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.