Ethidium Homodimer-1 (EthD-1)
Ethidium Homodimer-1 (EthD-1)
Invitrogen™

Ethidium Homodimer-1 (EthD-1)

The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound toRead more
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Catalog number E1169
Price (USD)
457.00
Each
Add to cart
Price (USD)
457.00
Each
Add to cart

The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (Excitation/emission maxima ∼528/617 nm).

We recommend using high-quality anhydrous DMSO for a stock concentration of 1 to 2 mM in DMSO/H2O 1:4 (v/v).



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For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorOrange
Excitation Wavelength Range528 nm
Dye TypeCell-Impermeant
For Use With (Application)Cell staining assays
For Use With (Equipment)Fluorescence Microscope
Quantity1 mg
Detection MethodFluorescence
DescriptionEthidium Homodimer-1 (EthD-1)
Emission617 nm
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeViability Indicator
SubCellular LocalizationNucleic Acids, Nucleus
Unit SizeEach
Contents & Storage
Store in freezer (-5°C to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.