ELISA Buffer Kit
ELISA Buffer Kit
Invitrogen™

ELISA Buffer Kit

The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are requiredRead more
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Catalog number CNB0011
Price (USD)
576.00
Each
Add to cart
Price (USD)
576.00
Each
Add to cart
The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are required to run an ELISA. The kit provides sufficient material for 10 ELISA plates.

Kit components:
• Coating Buffer A (10 mM phosphate buffer, pH 7.4)
• Coating Buffer B (50 mM carbonate buffer, pH 9.4)
• Assay Buffer (Blocking Buffer) (5X)
• Wash Buffer (25X)
• Stabilized TMB
• Stop Solution

View all available buffers and reagents for ELISA applications.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormConcentrate
For Use With (Application)ELISA
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
Coating Buffer A (100 mL): 1 bottle
Coating Buffer B (100 mL): 1 bottle
Assay Buffer (5x) (200 mL): 1 bottle
Wash Buffer (25x) (100 mL): 3 bottles
Stabilized Chromogen (25 mL): 4 bottles
Stop Solution (100 mL): 1 bottle. Store at 2°C to 8°C.

Frequently asked questions (FAQs)

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.