One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli
One Shot&trade; OmniMAX&trade; 2 T1<sup>R</sup> Chemically Competent <i>E. coli</i>
Invitrogen™

One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli

The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremelyRead more
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Catalog number C854003
Price (USD)
538.00
Each
Add to cart
Price (USD)
538.00
Each
Add to cart

The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format. OmniMAX 2 T1R is a versatile strain, perfect to use in all cloning applications, including Gateway technology and TOPO PCR cloning.

OmniMAX 2 T1R Chemically Competent E. coli cells carry the tonA (also known as fhuA) mutation, which confers resistance to T1 and T5 phage infection. The tonA genotype offers added security to valuable clones and libraries. This strain lacks the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)), allowing efficient transformation of highly methylated DNA. The OmniMAX 2 T1R strain has the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries the recA1 mutation, which helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation that increases plasmid DNA yield and quantity. The OmniMAX 2 T1R strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids). In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. Importantly, the ccdA gene present on the F´ episome is deleted, allowing negative selection of Gateway vectors as the strain is sensitive to the ccdB gene product.

One Shot OmniMAX 2 T1R Chemically Competent E. coli offer:
• Highest transformation efficiencies of >5 x 109 cfu/μg 
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Δ(ccdAB) for sensitivity to the toxic effects of the ccdB gene product, allowing negative selection of vectors containing the ccdB gene (Gateway cloning system)
• Stable F´ episome that carries lacIq repressor for inducible expression from trc, tac, and lac promoters
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• lacZΔM15 for blue-white color screening of recombinant clones 
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I 
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Easy-to-use One Shot format 
OmniMAX 2 T1R Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination.

Genotype
F´ [proAB+lacIqlacZΔM15 Tn10(TetR) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 (NalR) relA1 tonA panD

Find the strain and format that fit your needs
We offer other strains in chemically competent and electrocompetent cell formats to meet your specific needs.
OmniMAX 2 T1R and other strains are available in MultiShot formats for high throughput applications.
Explore bacterial growth media formats.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeChemically Competent Cells
Contains F' EpisomeYes
Improves Plasmid QualityYes (endA1)
Cloning Methylated DNAYes (mcrA)
Transformation Efficiency LevelHigh Efficiency (>1 x 109 cfu/μg)
Antibiotic Resistance BacterialYes (Tetracycline)
Cloning Unstable DNANot suitable for cloning unstable DNA
Blue/White ScreeningYes (lacZΔM15)
High-throughput CompatibilityLow
PlasmidMay be used for plasmids >20 kb
Preparing Unmethylated DNANo
Reduces RecombinationYes (recA1)
Shipping ConditionDry Ice
T1 Phage - Resistant (tonA)Yes
SpeciesE. coli (K12)
FormatTube
Product LineOne Shot™
Quantity21 x 50 μL
Unit SizeEach
Contents & Storage
• One Shot OmniMAX 2 T1R E. coli (21 x 50 μL)
Store Competent Cells at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.

• S.O.C. Medium (6 mL)
Store S.O.C. Medium at 4°C or room temperature.

Frequently asked questions (FAQs)

What strain should I use to transform my library?

OmniMAX 2 is the preferred strain for transforming libraries because of its high transformation efficiency and genomic cloning compatibility characteristics.

Do any Invitrogen competent cells contain DMSO in the freezing medium?

Yes, several of our competent cells products are frozen with DMSO. The presence of DMSO (dimethylsulfoxide) will generally be indicated in the MSDS files if you have a question about a particular product, but here is a list of commonly used products that are known to have DMSO in the freezing buffer:

One Shot OmniMAX 2 T1 Phage Resistant Cells, Cat. No. C8540-03

One Shot INV?F' Chemically Competent Cells, Cat. No. C2020-03 and C2020-06

One Shot MAX Efficiency DH5?-T1 Chemically Competent Cells, Cat. No. 12297-016

MAX Efficiency DH5?-T1 Phage Resistant Cells, Cat. No. 12034-013

MAX Efficiency DH5? Chemically Competent Cells, Cat. No. 18258-012

Library Efficiency DH5? Chemically Competent Cells, Cat. No. 18263-012

MAX Efficiency DH5? F'IQ Cells, Cat. No. 18288-019

MAX Efficiency Stbl2Chemically Competent Cells, Cat. No. 10268-019

Does the methylation status of DNA affect its ability to be cloned?

Yes. Bacterial host cells will often degrade incoming DNA that has a methylation pattern that is "foreign" relative to that of the cell. Several host strains have been modified to accept mammalian methylation patterns. The modified markers include mcrA, mcrBC, and mrr. Also, endogenous (b-type) restriction endonucleases can be problematic. Modifications of the host to be rK- or rB- are necessary and include hsdR17(AK-, MK+), hsdR17(rK-, mK-), hsdS20(rB-, rB-) or hsdRMS. Strains with the hsdR17(rK-, mK+) mutation lack K-type restriction endonuclease, but contain K-type methylase. DNA prepared from hosts that are rK- mK- is unmethylated and will transform with lower efficiency in rK+ hosts.

TOP10, DH10B, and OmniMAX2-T1 cells contain the mcr, mrr, and hsdRMS mutations. Mach1 and standard DH5? strains only have the hsdR17(rK- mK+) mutation and are not recommended for cloning eukaryotic genomic DNA.

Can encapsulated phagemid DNA or M13 phage be used to infect bacteria?

Single-stranded DNA viral particles like M13 require the presence of an F pilus in order to infect E. coli. This criterion is met by TOP10F', DH5? F'IQ, INV?F', Stbl4, OmniMAX2-T1 and DH12S cells. These cells are not traD mutants, which effectively allows the cells to retain the F' episome. Transforming single-stranded DNA can cause a 100- to 1,000-fold reduction in efficiency compared to viral particles.

Is S.O.C. medium absolutely required when recovering competent bacterial cells during transformation?

Many media can be used to grow transformed cells, including standard LB, SOB or TB broths. However, S.O.C. is the optimal choice for recovery of the cells before plating. The nutrient-rich formula with added glucose is often important for obtaining maximum transformation efficiencies.

View all One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli FAQs