CoroNa™ Green, AM, cell permeant - Special Packaging
CoroNa™ Green, AM, cell permeant - Special Packaging
Invitrogen™

CoroNa™ Green, AM, cell permeant - Special Packaging

CoroNa Green dye is a sodium ion indicator that exhibits an increase in green fluorescence emission intensity upon binding Na+,Read more
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Catalog number C36676
Price (USD)
796.00
Each
Add to cart
Price (USD)
796.00
Each
Add to cart
CoroNa Green dye is a sodium ion indicator that exhibits an increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength. Like our other sodium indicators, SBFI (Cat. nos. S1262, S1263, S1264) and Sodium Green dye (Cat. nos. S6900, S6901), the CoroNa Green indicator allows spatial and temporal resolution of Na+ concentrations in the presence of physiological concentrations of other monovalent cations. CoroNa Green AM dye loads into cells more efficiently than does Sodium Green tetraacetate, and the CoroNa Green indicator responds to a broader range of Na+ concentration. With absorption/emission maxima of ∼492/516 nm for the Na+-bound form, CoroNa Green dye can be detected by any instrument that can detect fluorescein. CoroNa Green dye is also available in a cell impermeant form (Cat. no. C36675).

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Application)Cell Viability and Proliferation
For Use With (Equipment)Fluorescence Microscope
Product TypeSodium Ion Indicator
Dye TypeSodium Indicator
Product LineCoroNa™
Quantity20 x 50 μg
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.