5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate
5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate
Invitrogen™

5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate

Carboxy SNARF™-1, acetoxymethyl ester, acetate is a cell-permeant pH indicator that has a pKa of ∼7.5 after de-esterification; thus, itRead more
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Catalog NumberQuantity
C127220 x 50μg
Catalog number C1272
Price (USD)
664.00
Each
Add to cart
Quantity:
20 x 50μg
Price (USD)
664.00
Each
Add to cart
Carboxy SNARF™-1, acetoxymethyl ester, acetate is a cell-permeant pH indicator that has a pKa of ∼7.5 after de-esterification; thus, it is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF™-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product TypepH Indicator
Dye TypepH Indicator
Product LineSNARF™
Quantity20 x 50μg
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.