NativePAGE™ Running Buffer (20X)
NativePAGE™ Running Buffer (20X)
Invitrogen™

NativePAGE™ Running Buffer (20X)

NativePAGE Running Buffer (20X) is used to make the NativePAGE Cathode and Anode running buffers for use with a MiniRead more
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Catalog number BN2001
Price (USD)
279.00
Each
Add to cart
Price (USD)
279.00
Each
Add to cart
NativePAGE Running Buffer (20X) is used to make the NativePAGE Cathode and Anode running buffers for use with a Mini Gel tank or an XCell SureLock Mini Cell when running NativePAGE gels. This buffer is critical for maintaining a near-neutral pH environment during electrophoresis, which is vital in maximizing the stability of both proteins and gel matrix.

The NativePAGE Gel System is a sophisticated electrophoresis system specifically designed for the analysis of native protein complexes. Unlike standard SDS-PAGE systems, the NativePAGE system maintains proteins in their native state without denaturation. This is achieved by using Coomassie G-250 as a charge-shift molecule, which binds to proteins and confers a net negative charge while retaining their native structure.

This system offers several advantages over traditional native PAGE systems such as Tris-Glycine and Tris-Acetate. Notably, it operates at a near-neutral pH and is compatible with detergents, providing greater stability and resolution for proteins during electrophoresis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferRunning Buffers
Concentration20 X
Gel TypeNative-PAGE
Product TypePAGE Running Buffer
Shelf Life1 Year
Gel CompatibilityNativePAGE™ Gels
Product LineNativePAGE™
Quantity1 L
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store at room temperature

Frequently asked questions (FAQs)

What gels can I use to separate native proteins?

The NativePAGE Invitrogen Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analyzing native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessing purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

After transferring onto a nitrocellulose membrane, what is the best way to store the membrane overnight for probing the next day?

We recommend rinsing the membrane briefly in water, air drying and store it at room temperature in a ziplock bag. Do not place nitrocellulose in the freezer because it will shatter. Unlike PVDF, nitrocellulose membranes should never be pre-wetted in alcohol as it will cause them to shrivel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I would like to run a NativePAGE gel. Which of your protein standards should I use?

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels?

We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels or NuPAGE Tris-Acetate gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NativePAGE™ Running Buffer (20X) FAQs