NativePAGE™ 3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gels
NativePAGE™ 3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gels
Invitrogen™

NativePAGE™ 3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gels

The Invitrogen NativePAGE Bis-Tris Gel System is a precast polyacrylamide mini-gel system that provides sensitive, high-resolution analysis of native proteins and protein complexes for molecular mass estimations, and assessment of purity.
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Catalog NumberWells
BN1001BOX10-well
BN1003BOX15-well
Catalog number BN1001BOX
Price (USD)
242.00
Each
Add to cart
Wells:
10-well
Price (USD)
242.00
Each
Add to cart
The Invitrogen NativePAGE Bis-Tris Gel System is a precast polyacrylamide mini-gel system that provides sensitive, high-resolution analysis of native proteins and protein complexes for molecular mass estimations, and assessment of purity. It is based on the blue native polyacrylamide gel electrophoresis (BN PAGE) technique developed by Schägger and von Jagow, which overcomes the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility.

Features of the NativePAGE Bis-Tris Gel System include:
• Wide molecular weight resolving range, from 15 – 10,000 kDa
• Neutral-pH separation, which better preserves the native state of protein complexes
• Resolution of all proteins in the gel regardless of their isoelectric point (pI)
• Ability to analyze membrane-protein complexes in their native conformations
• Ability to obtain better resolution than with traditional tris-glycine native electrophoresis

How NativePAGE Bis-Tris Gels work
In SDS-PAGE, SDS functions as a charge-shift molecule that denatures proteins by conferring on them a net negative charge and enables proteins to migrate towards the anode. BN PAGE uses Coomassie G-250 as the charge-shift molecule, which binds to proteins and confers a net negative charge while maintaining the proteins in their nondenatured, native state. The near-neutral pH of the NativePAGE Bis-Tris Gel System provides maximum stability of both the proteins and the gel matrix, resulting in a highly sensitive method for analysis of native membrane-protein complexes and offering superior band resolution over traditional Tris-glycine gel systems.

For transferring proteins to a membrane, we recommend using NuPAGE Transfer Buffers for traditional wet transfer and PVDF membranes. The NuPAGE transfer buffer maintains the neutral pH environment established during electrophoresis. Nitrocellulose membranes are not compatible for blotting since the membrane will bind the Coomassie G-250 dye very tightly. Alternatively, Rapid semi-dry transfer can be done using the Invitrogen Power Blotter (PB0013) or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001) using PVDF membranes.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage3 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsNative
Sample Loading VolumeUp to 25 μL
Separation Range30 to 10,000 kDa
Separation TypeNative
Shelf Life12 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineNativePAGE™
Quantity10 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit SizeEach

Frequently asked questions (FAQs)

What gels can I use to separate native proteins?

The NativePAGE Invitrogen Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analyzing native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessing purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What does it mean when bands appear to be getting narrower (or "funneling") as they progress down a protein gel?

There may be too much beta-mercaptoethanol (BME), sample buffer salts, or dithiothreitol (DTT) in your samples. If the proteins are over-reduced, they can be negatively charged and actually repel each other across the lanes causing the bands to get narrower as they progress down the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

After transferring onto a nitrocellulose membrane, what is the best way to store the membrane overnight for probing the next day?

We recommend rinsing the membrane briefly in water, air drying and store it at room temperature in a ziplock bag. Do not place nitrocellulose in the freezer because it will shatter. Unlike PVDF, nitrocellulose membranes should never be pre-wetted in alcohol as it will cause them to shrivel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are NativePAGE gels and buffers compatible with Mini Gel tank?

Yes, NativePAGE gels are compatible with our Mini Gel tank, however there is a small variation from the original protocol.

The following protocol are for using NativePage gels with the Mini Gel Tank depending on if you are also performing a western transfer or not:
If you are NOT performing a western transfer:
1. Prepare 250 mL of each buffer (Anode and Dark Blue Cathode) per gel
2. After inserting a loaded NativePAGE gel into the Mini Gel Tank and pulling the clamp forward, fill the front cathode buffer chamber to the Fill Line with Dark Blue Cathode Buffer (~200 mL per gel)
3. Add 220 mL of Anode Buffer to the back anode buffer chamber for that gel. Start the gel run

If you are performing a western transfer:
1. Prepare 250 mL of Dark Blue Cathode Buffer, 250 mL of Light Blue Cathode Buffer, and 500 mL of Anode Buffer per gel
2. After inserting a loaded NativePAGE gel into the Mini Gel Tank and pulling the clamp forward, fill the front cathode buffer chamber to the Fill Line with Dark Blue Cathode Buffer (~200 mL per gel
3. Add 220 mL of Anode Buffer to the back anode buffer chamber for that gel
4. Start the gel run; pause the run after the dark blue dye has run ~1/3 of the way through gel
a. Pour out the buffers from the Mini Gel Tank
b. Refill the back anode buffer chamber with 220 mL of Anode Buffer per gel
c. Fill the front cathode buffer chamber to the Fill Line with Light Blue Cathode Buffer (~200 mL per gel)
5. Resume the gel run

Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .

I would like to run a NativePAGE gel. Which of your protein standards should I use?

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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