Biotin-XX phalloidin is a high-affinity F-actin probe conjugated to biotin-XX.• Selectively stains F-actin• Superior to antibody staining• Optimal for fixedRead more
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Catalog number B7474
Price (USD)
588.00
Each
Add to cart
Price (USD)
588.00
Each
Add to cart
Biotin-XX phalloidin is a high-affinity F-actin probe conjugated to biotin-XX.
• Selectively stains F-actin • Superior to antibody staining • Optimal for fixed and permeabilized samples
Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Demonstrating very little nonspecific staining, Biotin-XX phalloidin allows high-contrast discrimination of actin staining.
Use in Multiple Applications Biotin-XX phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Biotin-XX phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot™ nanocrystals, and Alexa Fluor™ conjugates including secondary antibodies.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Dye TypeBiotin-XX
Quantity50 units
Shipping ConditionRoom Temperature
Label TypeBiotin and Other Haptens
Product TypePhalloidin
SubCellular LocalizationActin, Cytoskeleton
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C.
Frequently asked questions (FAQs)
I want to label F-actin with phalloidin and image with transmission electron microscopy. What is the best way to go about it?
One way is to use a biotin-phalloidin conjugate, then label that with a colloidal gold conjugate of streptavidin. You will need to block endogenous biotin first. Another method is to label with fluorescein phalloidin, then with an anti-fluorescein antibody conjugated with colloidal gold.
I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?
When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.