BackDrop™ Background Suppressor for Microplate Assays
BackDrop™ Background Suppressor for Microplate Assays
Invitrogen™

BackDrop™ Background Suppressor for Microplate Assays

BackDrop™ Background Suppressor is a novel reagent designed to effectively suppress background fluorescence in live cell assays. It helps improveRead more
Have Questions?
Catalog NumberIncludes
B10512One 50 mL bottle
Catalog number B10512
Price (USD)
476.00
Each
Add to cart
Includes:
One 50 mL bottle
Price (USD)
476.00
Each
Add to cart
BackDrop™ Background Suppressor is a novel reagent designed to effectively suppress background fluorescence in live cell assays. It helps improve signal-to-noise ratios when used with green-emitting fluorescent dyes or proteins.

• Can be added to complete media
• Improves sensitivity of green-emitting fluorescent dyes or proteins
• Validated with many cellular assays, including ion indicator and membrane potential assays
• Minimal cell toxicity—demonstrated to not cause osmotic shock to neuronal cells

The sensitivity of fluorescent assays can be compromised by autofluorescence or background fluorescent signals. These background signals result from cellular proteins or growth media components and contribute to a decrease in the signal-to-noise ratio and reduced assay sensitivity. Addition of BackDrop™ Background Suppressor significantly reduce background signal, helping improve experimental results.

BackDrop™ Background Suppressor can be added to growth media and has been formulated to not contribute to cell toxicity. It has been successfully used with many different cell types and fluorescent reagents (including ion indicators) to efficiently suppress background fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
IncludesOne 50 mL bottle
Quantity50 mL
FormLiquid
Product LineBackDrop™
For Use With (Application)Microplate Assays
Product TypeBackground Suppressor for live cell assays
Unit SizeEach
Contents & Storage
Store at 2-8°C. DO NOT FREEZE. Protect material from long-term exposure to light.

Frequently asked questions (FAQs)

How do I quench any fluorescence outside of my live cells and leave my intracellular staining intact?

There are a number of reagents used to quench fluorescence on the outside of live cells or in the media (background fluorescence). Our Backdrop Background Suppressor reagents are a great option to quench blue, green, and red fluorescent dyes, various media components and fluorescent proteins. Another commonly used reagent is the cell impermeant colorimetric dye, Trypan blue.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The BackDrop background suppressor solutions are supposed to reduce background fluorescence. Does that include sample autofluorescence?

No. The BackDrop dyes will quench any fluorescence of a given wavelength in the extracellular media or buffer, but not inside live cells. It will not quench autofluorescence inside live cells unless the dye is engulfed.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the Fluo-4 Calcium Imaging Kit (Cat. No. F10489) components be purchased separately?

Yes. The following components are sold separately:
Component B: PowerLoad Concentrate (Cat. No. P10020)
Component C: BackDrop Background Suppressor for Microplate Assays (Cat. No. B10512)
Component D: Probenecid, Water Soluble (Cat. No. P36400)

Can I use the BackDrop Background Suppressor reagent as the quencher to reach the inner leaflet of the plasma membrane?

We have not tested this. The BackDrop Background Suppressor reagent is cell impermeant, but if the quenching is caused by a FRET interaction and, if the distance between the BackDrop Background Suppressor outside of the cell is within the Ro distance for FRET with the fluorescent dye in the inner leaflet of the membrane, quenching may still occur.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Would the BackDrop Background Suppressor reagent quench Alexa Fluor 647 signal? What is the mechanism of quenching?

Yes, the BackDrop Background Suppressor reagent can be used to quench Alexa Fluor 647 signal. We have not determined the exact mechanism for the quenching but it depends upon the dye. For some dyes, it may be a FRET interaction, for others the BackDrop Background Suppressor absorbs the photons emitted by the fluorescent dye (inner filter effect).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all BackDrop™ Background Suppressor for Microplate Assays FAQs