About the Mini Blot Module The Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Mini Blot Module is compatible with Bolt™ Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Bolt™
Quantity≤ 2 Blot Modules/Mini-Gel Tank, 1 Mini-Gel/Blot Module
MaterialPVDF
Mode of TransferWet
Dimensions (LxW)Mini, 8 cm x 8 cm
Product LineBolt
Unit SizeEach
Contents & Storage
2 Mini Blot Modules 125 mL Bolt™ Transfer Buffer (20X) 15 mL Bolt™ Antioxidant 20-pack PVDF membranes (0.2 μm)
Frequently asked questions (FAQs)
Can I prepare my protein sample with the reducing agent and store it for future use?
DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?
Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.