10X Bolt™ Sample Reducing Agent
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Invitrogen™

10X Bolt™ Sample Reducing Agent

Bolt Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitolRead more
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Catalog NumberQuantity
B0004250 μL
B000910 mL
Catalog number B0004
Price (USD)
19.59
Each
Add to cart
Quantity:
250 μL
Price (USD)
19.59
Each
Add to cart
Bolt Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitol (DTT) for a 10X concentration in a stabilized liquid form.

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10X, 500 mM
For Use With (Equipment)Bolt Mini Gel Tank
Product TypeSample Reducing Agent
FormLiquid
Formulation500 mM dithiothreitol (DTT)
Gel CompatibilityBolt™ Bis-Tris Plus Gels
Product LineBolt™
Quantity250 μL
Reagent TypeReducing Agent
Unit SizeEach
Contents & Storage
Sample Reducing Agent (10X) contains 500 mM dithiothreitol (DTT).

Store at 2°C to 8°C.

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

View all 10X Bolt™ Sample Reducing Agent FAQs