MagMAX™-96 Viral RNA Isolation Kit
MagMAX™-96 Viral RNA Isolation Kit
Applied Biosystems™

MagMAX™-96 Viral RNA Isolation Kit

The MagMAX-96 Viral RNA Isolation Kit is for the isolation of viral RNA and DNA from diverse sample types (e.g.,Read more
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Catalog NumberNo. of Reactions
AM183696
AMB18365480
Catalog number AM1836
Price (USD)
586.00
Each
Add to cart
No. of Reactions:
96
Price (USD)
586.00
Each
Add to cart
The MagMAX-96 Viral RNA Isolation Kit is for the isolation of viral RNA and DNA from diverse sample types (e.g., environmental samples and biofluids). Nucleic acids purified using this kit are ideal for PCR and RT-PCR applications. For a NVSL-validated version of this kit, please see the MagMAX-96 AI⁄ND Viral RNA Isolation Kit.

Benefits of MagMAX magnetic bead-based purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 μL of nuclease-free water, concentrating RNA from large, dilute samples.

Optimized for isolation of virus from biofluids
The MagMAX Viral RNA Isolation Kit has been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity. Without the need for organic solvents or RNA precipitation, the MagMAX Viral RNA Isolation Kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields. With this kit, DNA and RNA input levels ranging from 20 copies to 25 x 106 copies have been quantitatively recovered from plasma, serum, and milk. And even with less sample input, pathogen detection was as sensitive (equivalent CTs) or more sensitive (lower CTs) using nucleic acid isolated with the MagMAX kit when compared to nucleic acid isolated using another company's product.

Accessory Products:
This kit is optimized for automated nucleic acid purification using KingFisher magnetic particle processors. This kit can also be used for manual nucleic acid purification using a 96-well magnetic ring stand. The Magnetic Stand-96 (Cat. No. AM10027) and the 96-well Magnetic Ring Stand (Cat. No. AM10050) are designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates. The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors.
For Veterinary Use Only.
Specifications
Format96-well Plate
Isolation TechnologyMagnetic Bead
Purification Time45 min.
Sample TypeCell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples
Final Product TypeViral DNA⁄RNA
High-throughput CompatibilityHigh-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Equipment)MagMAX™ Express-96 Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
For Use With (Application)Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
No. of Reactions96
Product LineMagMAX™
ScaleMidi
Starting Material AmountUp to 50μl of sample
TargetViral DNA/RNA
Yield5 ng
Unit SizeEach
Contents & Storage
Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature.

Frequently asked questions (FAQs)

I need a recommendation for a MagMAX kit for automated viral RNA isolation from Animal Health samples such as serum, nasal fluid, and swab samples. What would you recommend?

Cat. No. AM1836 is a good choice if you want to process 50 µL of samples. If you want to maximize the input amount and use more than that (up to 300 µL) we recommend using the MagMAX RNA/DNA Pathogen kit (Cat. No. 4462359). The buffers and reagents included in these MagMAX kits are identical, but the volumes are larger for the MagMAX Pathogen RNA/DNA kit allowing for more sample input.

Is the the direct lysis approach to DNA/RNA analysis applicable for extraction of viral RNA?

Some customers have isolated RNA from viruses. Unfortunately, the protein coat could cause problems. Another option would be to use the MagMax Viral Isolation Kit.

Are MagMax kits compatible with RNAlater reagent-treated samples?

Only MagMax96 for Microarrays Total RNA Isolation Kit (Cat. No. AM1839) is compatible, since it uses Trizol reagent and most of the salt carry-over from RNAlater reagent is eliminated during the lysis steps and partitioned from the aqueous RNA layer on top. For other MagMax kits, optimization is needed. High salt carry over is not optimal for MagMax RNA isolation steps.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Can I edit the manufactured KingFisher Flex protocols?

No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.

Can I use magnetic beads from other vendors with the KingFisher Flex instrument?

For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.