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Retic Lysate IVT™ Kit
The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimizedRead more
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| Catalog Number | Product Type | Quantity |
|---|---|---|
| AM1200 | Retic Lysate | 60 Reactions |
| AM1200M | Retic Lysate | 60 Reactions |
Catalog number AM1200
Price (USD)
894.00
Each
Product Type:
Retic Lysate
Quantity:
60 Reactions
Price (USD)
894.00
Each
The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimized lysate results in high protein yield, biological activity, and consistent performance. Advantages of the Retic Lysate IVT™ Kit:
• Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs
• Accommodates large volume of template mRNA
How it works
The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA. Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences. A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. The Retic Lysate IVT™ Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG.
Reference
1. Kozak, M. (1990) Evaluation of the fidelity of initiation of translation in reticulocyte lysates from commercial sources. Nucleic Acids Res 18:2828.
• Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs
• Accommodates large volume of template mRNA
How it works
The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA. Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences. A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. The Retic Lysate IVT™ Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG.
Reference
1. Kozak, M. (1990) Evaluation of the fidelity of initiation of translation in reticulocyte lysates from commercial sources. Nucleic Acids Res 18:2828.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Expressed Protein TypeWater Soluble Protein
Expression MechanismCell-Free Expression
Expression SystemCell-Free
For Use With (Application)In Vitro Transcription
No. of Reactions60 Reactions
Product LineAmbion™
Product TypeRetic Lysate
Quantity60 Reactions
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
• 5 × 200 μl (store at -80°C)
• 2 × 1.75 ml Nuclease-free Water (store at any temperature)
• 100 μl 20X Translation Mix minus-Met
• 100 μl 20X Translation Mix minus-Leu
• 100 μl High Salt Mix minus-Met
• 100 μl Low Salt Mix minus-Met
• 50 μl Capped Xef-1 RNA (50 μg/ml)
Unless otherwise stated, store reagents below -70°C. Do not store cell-free translation systems in the liquid phase of a liquid nitrogen storage system.
• 2 × 1.75 ml Nuclease-free Water (store at any temperature)
• 100 μl 20X Translation Mix minus-Met
• 100 μl 20X Translation Mix minus-Leu
• 100 μl High Salt Mix minus-Met
• 100 μl Low Salt Mix minus-Met
• 50 μl Capped Xef-1 RNA (50 μg/ml)
Unless otherwise stated, store reagents below -70°C. Do not store cell-free translation systems in the liquid phase of a liquid nitrogen storage system.