CBQCA Plus Protein Quantitation Kit
CBQCA Plus Protein Quantitation Kit
Invitrogen™

CBQCA Plus Protein Quantitation Kit

Green features
The CBQCA Plus Protein Quantitation Kit is a specialized tool designed for the accurate quantitation of proteins in solution. ThisRead more
Have Questions?
Catalog number A66522
Price (USD)
396.00
Each
Add to cart
Price (USD)
396.00
Each
Add to cart

The CBQCA Plus Protein Quantitation Kit is a specialized tool designed for the accurate quantitation of proteins in solution. This kit is a refined version of the CBQCA Protein Quantitation Kit (Cat. No. C6667), with a significant modification: potassium cyanide, a toxic compound, has been replaced with mandelonitrile, a less-toxic alternative. This change does not compromise the kit's performance, maintaining the same level of sensitivity and accuracy as its predecessor.

The CBQCA Plus Protein Quantitation Kit exhibits high sensitivity and is capable of detecting as little as 50 ng of bovine serum albumin (BSA) in an assay volume of 100–200 μL. This sensitivity allows for precise measurements even in low-protein samples. Moreover, the kit is compatible with detergents, a feature that enhances its versatility. This compatibility makes it suitable for accurate quantitation of proteins in the presence of lipids, membrane fractions, or detergents, as well as for lipoproteins and small peptides.

The kit includes all necessary components for performing 300–800 assays, depending on the concentration of substrate used. The resulting fluorescent product is maximally excited at 465 nm and has an emission maxima at 550 nm. It is designed for use with a fluorescence microplate reader, but can also be adapted for use with a standard fluorometer or minifluorometer after dilution of the reaction mixture.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Green FeaturesLess hazardous
Assay Range50 ng to 150 μg
AssayFluorescent Protein Assay
Detection MethodFluorescence
Product TypeProtein Quantitation Assay
For Use With (Application)Quantitating proteins in solution
For Use With (Equipment)Microplate Reader, Fluorometer
Quantity1 Kit
Shipping ConditionWet Ice
FormatKit
Excitation/Emission465/550 nm
Unit SizeEach
Contents & Storage
Sufficient for: 300 to 800 assays

• Component A: ATTO-TAG CBQCA derivatization reagent, 5 mg
• Component B: Dimethylsulfoxide (DMSO), 1 mL
• Component C: Mandelonitrile, 20 mM in 4 mL DMSO
• Component D: Bovine serum albumin (BSA) standard, 4 mg
• BupH Borate Buffer Pack

Store in freezer (-5°C to -30°C) and protect from light.

Frequently asked questions (FAQs)

What is the difference between the CBQCA Plus Protein Quantitation Kit (Cat. No. A66522) and the CBQCA Protein Quantitation Kit (Cat. No. C6667)?

There are two main differences between the two kits. Firstly, the CBQCA Protein Quantitation Kit (Cat. No. C6667) contains a potassium cyanide (KCN) component (component C). This component has been removed in the CBQCA Plus Protein Quantitation Kit (Cat. No. A66522) and replaced with mandelonitrile which serves as a less toxic alternative to KCN without compromising assay performance. Secondly, the CBQCA Plus Protein Quantitation Kit includes one additional component compared to the CBQCA Protein Quantitation Kit, a BupH Borate Buffer Pack.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What is the shelf life of the BupH Borate Buffer Pack after reconstitution?

When properly reconstituted using a clean water source and good handling technique, the BupH Borate Buffer is stable for approximately 3-6 months when stored at 4 degrees C. For longer-term stability, we recommend adding sodium azide to a final concentration of 0.05% (w/v).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What is the working range for the CBQCA Plus Protein Quantitation Kit?

The working range for the CBQCA assay is 50 ng - 150 µg protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

The CBQCA Plus Protein Quantitation Kit was shipped on wet ice but the storage recommendation is at -20 degrees C? Is it still good to use?

Yes, the product is still good to use. We ship many of our products at a higher temperature than the storage recommendation. The product will be stable for at least one week at the shipping temperature when in the original, unopened packaging. For long-term stability, the product should be placed at the recommended storage temperature upon receipt.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Can I use the CBQCA Plus Protein Quantitation Kit to quantitate small peptides?

We do not recommend using the CBQCA assay for quantitating small peptides since the BSA standard used is not reflective of a peptide sample. A better assay to use would be the Pierce Quantitative Fluorometric Peptide Assay (Cat. No. 23290) or the Perce Quantitative Colorimetric Peptide Assay (Cat. No. 23275).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

View all CBQCA Plus Protein Quantitation Kit FAQs