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EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, whichRead more
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| Catalog Number | Quantity |
|---|---|
| A65393 | 100 Reactions |
| A65394 | 500 Reactions |
Catalog number A65393
Price (USD)
377.00
Each
Quantity:
100 Reactions
Price (USD)
377.00
Each
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Features of the EquiPhi29 DNA Amplification Kit include:
• Fast DNA amplification in less than two hours
• Sensitivity from 1 fg of input DNA
• Maximal yield of 17 µg amplified DNA
• Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies)
• Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression
• All required components for DNA amplification in RCA or MDA-WGA reactions
The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias. Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs. The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeDNA Amplification Kit
FormLiquid
For Use With (Application)RCA, MDA-WGA
PolymeraseEquiPhi29 DNA Polymerase
Quantity100 Reactions
Research CategoryNucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
FormatKit
Optimal Reaction Temperature42°C
Shipping ConditionDry Ice
Reaction TimeUp to 2 hours
Unit SizeEach
Contents & Storage
• EquiPhi29 DNA Polymerase (0.1 mL at 10 U/μL)
• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1 mL)
• 0.1 M DTT (0.25 mL)
• 500 μM Exo-Resistant Random Primer (0.2 mL)
• 10 mM dNTP Mix (0.2 mL)
• 0.1 U/μL Pyrophosphatase, inorganic (0.1 mL)
Store at -15 to -25°C.