TMT11-131C Label Reagent
TMT11-131C Label Reagent
Thermo Scientific™

TMT11-131C Label Reagent

Tandem mass tag (TMT) reagents are specially designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberDescription
A58333TMT11plex Label Reagents ​in stabilized solution, ​96-well plate, 8 x 40 μg​ (per tag)
A34807TMT11-131C Label Reagent, 1 x 5 mg
A37724TMT11-131C Label Reagent, 3 x 0.8 mg
A34808TMT10plex Isobaric Label Reagent Set plus TMT11-131C Label Reagent, 1 x 5 mg (per tag)
A37725TMT10plex Isobaric Label Reagent Set plus TMT11-131C Label Reagent, 3 x 0.8 mg (per tag)
Catalog number A58333
Price (USD)
640.00
Each
Add to cart
Description:
TMT11plex Label Reagents ​in stabilized solution, ​96-well plate, 8 x 40 μg​ (per tag)
Price (USD)
640.00
Each
Add to cart
Tandem mass tag (TMT) reagents are specially designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT11-131C tag structure is identical to the other TMT10pex tags, but the TMT11-131C mass reporter is labeled with only 13C isotopes. The addition of the TMT11-131C tag enables separation of the 131-mass reporter ion of the original TMT10plex reagents into N and C variants, creating an 11th channel for relative quantitation using high resolution Orbitrap MS instruments.

The advantages of TMT label reagents include increased multiplexed relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex label reagents plus TMT11-131C is ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or to improve statistics on biological replicates.

Features of this TMT11-131C Label Reagent include:
• Multiplex—concurrent MS analysis of up to 11 samples derived from cells, tissues, or biological fluids
• Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
• Efficient—amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
• Compatible—optimized for use with high resolution Thermo Scientific Orbirtrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer 2.3

TMT11-131C is available as a standalone tag (Cat. No. A34807, A37724), as part of this TMT10plex Isobaric Label Reagent Set plus TMT11-131C, or as part of the complete 11plex set of reagents in a 96-well plate format. When combined with the industry-leading, high resolution Orbitrap instruments and software, TMT reagents provide integrated total solutions for quantitative protein expression analysis.

Specifications
DescriptionTMT11plex Label Reagents ​in stabilized solution, ​96-well plate, 8 x 40 μg​ (per tag)
FormulationLiquid in stabilized DMSO solution
For Use With (Equipment)Mass Spectrometer
Product TypeIsobaric Label Reagent Set
Product LineTMT10plex™
Label or DyeTandem Mass Tag™ (TMT) Labels
Starting MaterialCell Lysate
Final Product TypeProtein PEPTIDE
Detection MethodMass Spectrometry
Workflow StepProtein Labeling
Quantity88 Reactions
FormLiquid
Unit SizeEach
Contents & Storage

Sufficient for: Eight 11-plex (8 x 11-way) experiments
• TMT11-126 Label Reagent, 8 x 40 μg
• TMT11-127N Label Reagent, 8 x 40 μg
• TMT11-127C Label Reagent, 8 x 40 μg
• TMT11-128N Label Reagent, 8 x 40 μg
• TMT11-128C Label Reagent, 8 x 40 μg
• TMT11-129N Label Reagent, 8 x 40 μg
• TMT11-129C Label Reagent, 8 x 40 μg
• TMT11-130N Label Reagent, 8 x 40 μg
• TMT11-130C Label Reagent, 8 x 40 μg
• TMT11-131N Label Reagent, 8 x 40 μg
• TMT11-131C Label Reagent, 8 x 40 μg

Store at -20°C.

Frequently asked questions (FAQs)

How can I tell if the buffer in which my peptides are resuspended are compatible with TMT and TMTpro labels?

TMT and TMTpro labels are not compatible with buffers containing primary amines. After protein digestion, peptides need to be cleaned up and dried down. Prior to labeling, we do recommend resuspending the peptides in 10 µL of either 100 mM TEAB (pH 8.5) or 100 mM HEPES (pH 8.0).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Is the TMT stabilizing solution compatible with subsequent MS steps?

For further steps after TMT labelling, the stabilizing solution can be considered as a 33% DMSO solution. If subsequent excess TMT removal will be performed using EasyPep solid-phase extraction (SPE), the DMSO solution does not need to be diluted. On the other hand, the use of any reverse phase chromatography method (i.e., Cat. No. 84868) might need dilution of the sample to ensure that the DMSO concentration is tolerated by the resin. Alternatively, the peptide mix can be dried down using Speed Vac and then resuspended in the corresponding buffer for a particular column (Reverse Phase or Desalting).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

With TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates, how much protein can be used per TMT or TMTpro reaction?

TMT and TMTpro label reagents in 96-well plates are optimized to work with 1 to 10 µg of protein digest.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can the sealing foil be partially removed with TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates?

Yes, the sealing foil can be partially peeled to access individual sets of TMT or TMTpro labels. Alternatively, each of the wells can be perforated to transfer the TMT/TMTpro labels to a different tube, if needed.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I determine the correct orientation of the TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates?

All the plates are barcoded in the long lower edge, helping to recognize correct plate orientation, plus the plate has a notch on the left corners.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all TMT11-131C Label Reagent FAQs