Crossflow Y-Connector
Crossflow Y-Connector
Thermo Scientific™

Crossflow Y-Connector

In order to filter sample volumes larger than 3 liters (up to 6 liters), two Pierce Crossflow Filtration Cassettes can be connected using the Y connector accessory, which is supplied with the appropriate tubing sizes and fittings.
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Catalog number A57936
Price (USD)
15.45
Each
Add to cart
Price (USD)
15.45
Each
Add to cart
In order to filter sample volumes larger than 3 liters (up to 6 liters), two Pierce Crossflow Filtration Cassettes can be connected using the Y connector accessory, which is supplied with the appropriate tubing sizes and fittings. The tubing size required for connecting the cassettes is 16, while the size needed for the pump is 15. A pump head size of 15 is required for parallel running of two cassettes.
• The Y-connector connects the sample feed line to two Pierce Crossflow Cassettes
• Two cassettes are needed to process sample volumes ranging from 3–6 L.
Specifications
Product TypePlastic Hose Connection
AutoclavableNot Autoclavable
MaterialPVC
Overall Length (English)4 ft.
Overall Length (Metric)122 cm
SterilityNon-sterile
Thread SizeConnects size 15 hose to two size 16 hoses
Unit SizeEach

Frequently asked questions (FAQs)

Are Pierce Crossflow Filtration Cassettes GMP grade?

Pierce Crossflow Filtration Cassettes are not GMP grade.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I run Pierce Crossflow Filtration Cassettes without the pressure gauge?

A pressure gauge is provided with every Pierce Crossflow Filtration Cassette. Pierce Crossflow Filtration Cassettes can be run without a pressure gauge; however, it is important to limit the flow rate to 200-300 mL/min to prevent tearing and potential collapse of the tubing. The flow rate can be determined by measuring the volume flow over 1 min. Alternatively, a pump speed of 300-350 rpm or a pressure of 1-1.5 bar will maintain a flow rate of 200-300 mL/min.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the factors that can speed up or slow down the rate of concentration or diafiltration?

High protein concentration and/or presence of particulate in the sample can slow down the rate of concentration/diafiltration. Adsorption of the sample to the membrane and lower temperature (4 degrees C vs room temperature) can also slow down concentration/diafiltration. Filtering your sample prior to processing with a crossflow filtration cassette will help speed up concentration/diafiltration.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the advantages of using a crossflow ultrafiltration device vs. a stirred cell device?

Crossflow ultrafiltration and stirred cell devices both effectively concentrate biomolecules with similar sample recoveries; however, a crossflow filtration device concentrates sample much faster than a stirred cell device.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why should I use crossflow filtration instead of dialysis or chromatography methods?

Crossflow filtration is much faster for concentration and diafiltration than dialysis and chromatography methods.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Crossflow Y-Connector FAQs