Efficient-Pro Medium is a next-generation Chinese hamster ovary (CHO) medium used in the production of monoclonal antibodies (mAbs), offering significantRead more
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Quantity
A5322203
20 L
A5322201
1000 mL
A5322202
10 L
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Catalog number A5322203
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1,398.00
Each
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Quantity:
20 L
Price (USD)
1,398.00
Each
Add to cart
Efficient-Pro Medium is a next-generation Chinese hamster ovary (CHO) medium used in the production of monoclonal antibodies (mAbs), offering significant performance and ease-of-use improvements over earlier Gibco and competitor products. Designed to work optimally with Efficient-Pro Feed 1 and Efficient-Pro Feed 2 for use in CHO-K1 and DG-44/CHO-S cell lines respectively, the Efficient-Pro system increases titer and specific productivity, improves protein quality and metabolite profiles, and is easier to use with less solubility issues than other CHO media and feed offerings.
The Efficient-Pro system will help you: • Sustain cell viability for longer bioreactor runs and maximized titer • Improve downstream outputs such as protein glycosylation, charge-variants, and aggregation • Speed up process development and streamline transfer to manufacturing scale • Increase flexibility with a range of format and packaging configurations • Decrease cell shock with simplified adaptation protocols
Efficient-Pro Medium has been formulated as a chemically defined, animal-origin-free cell culture medium used in the bioproduction of mAbs and other therapeutics in traditional batch and fed-batch bioprocessing workflows, particularly in combination with CHO cell line variants.
Efficient-Pro Medium is a balanced formulation designed to meet the metabolic needs of mAb production and does not contain growth factors or insulin to ensure batch-to-batch consistency and production efficiency. Demonstrated to improve productivity in a CHO-K1 cell line when paired with Efficient-Pro Feed 1 compared to an industry-leader’s media and feed system, the Efficient-Pro system helps accelerate your time-to-market and achieve success.
For research use only. Not for use in diagnostic procedures.
If my cell line is sensitive to ammonia, can I still use Efficient-Pro Medium?
Yes, however, we recommend reducing the L-glutamine concentration if a decrease in cell viability is observed. The recommended glutamine concentration is 2-8 mM. Another way to reduced ammonia production is to use GlutaMAX Supplement instead of L-glutamine. Addition of Pluronic F-68 is not required.
Does Efficient-Pro Medium require supplementation for use?
When using a CHO cell line with a selection system other than the glutamine synthetase (GS) system, we recommend adding 2-8 mM L-glutamine or GlutaMAX Supplement to Efficient-Pro Medium. Additionally, we recommend making sure that glucose levels do not fall below 2 g/L in culture by supplementing with glucose as needed. Also, if clumping becomes an issue, then add anti-clumping agent (ACA) to your cultures at 1% v/v.
Can CHO cells that are created or frozen in other production media be thawed into Efficient-Pro Medium or adapted for growth and production in Efficient-Pro Medium?
Cells that are grown in or adapted to, and frozen in other cell culture media readily adapt to Efficient-Pro Medium. However, if your cells are experiencing a significant lag or delay in growth, please directly adapt the cells into Efficient-Pro Medium and passage them for a minimum of 3 passages, until a consistent doubling time is observed.