Fetal Bovine Serum, Value, heat inactivated (formerly USDA-approved in North America or qualified, Brazil in other regions)
Fetal Bovine Serum, Value, heat inactivated (formerly USDA-approved in North America or qualified, Brazil in other regions)
Fetal Bovine Serum, Value, heat inactivated (formerly USDA-approved in North America or qualified, Brazil in other regions)
Fetal Bovine Serum, Value, heat inactivated (formerly USDA-approved in North America or qualified, Brazil in other regions)
Fetal Bovine Serum, Value, heat inactivated (formerly USDA-approved in North America or qualified, Brazil in other regions)
Gibco™

Fetal Bovine Serum, Value, heat inactivated (formerly USDA-approved in North America or qualified, Brazil in other regions)

Green features
Gibco Serum DeliversISO 13485-certified, processed in FDA-registered facilitiesTriple filtered at 0.1 μmGibco bottle is easier to use in hood, reducesRead more
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Catalog NumberQuantityFormat
A5256801500 mLBottle
A520950150 mLOne Shot
A520950210 x 50 mLOne Shot
Catalog number A5256801
Price (USD)
548.00
Each
Add to cart
Quantity:
500 mL
Format:
Bottle
Price (USD)
548.00
Each
Add to cart

Gibco Serum Delivers

  • ISO 13485-certified, processed in FDA-registered facilities
  • Triple filtered at 0.1 μm
  • Gibco bottle is easier to use in hood, reduces risk of contamination, and helps you perform cell culture more consistently
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

All products may not be available in all regions due to importation regulations

US Department of Agriculture (USDA) safety tested for the absence of bluetongue and akabane from countries that are USDA-approved for importation into the US

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that help you meet your research needs and budget requirements.

  • Sera category: Value
  • Sera application: Cell culture with robust cell lines, good value for basic research
  • Testing: Up to 30 quality tests
  • Endotoxin level: ≤20 EU/mL (levels routinely ≤10 EU/mL)
  • Hemoglobin level: ≤25 mg/dL
  • Origin: Refer to CoA for specific origin
Specifications
AgeFetal
SpeciesCattle/Bovine
Country of OriginRefer to CoA for specific origin
FormatBottle
Product TypeFetal Bovine Serum
Quantity500 mL
Serum TreatmentHeat-inactivated
SterilitySterile
Sterilization MethodTriple-filtered, 0.1 μm
GradeValue
Tested ForUp to 30 quality tests including 9CFR virus testing* endotoxin, performance (*Please note: If manufactured in the UK, Full 9CFR except for Rabies and BlueTongue Virus which is tested via EMA)
Endotoxin Concentration≤20 EU/mL
Green FeaturesLess waste
Heat InactivatedYes
Hemoglobin Concentration≤25 mg/dL
Purity or Quality GradeValue
Shelf Life5 Years
Shipping ConditionDry Ice
USDA Safety Testing IncludedUSDA Safety Testing Performed for Mexican and Australian origins
Unit SizeEach
Contents & Storage
Store at <-10°C.

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Can you please suggest an alternative to Fetal Bovine Serum, qualified, heat-inactivated, Canada, 500 mL (Cat. No. 12484028) that has been discontinued?

The alternative product is Fetal Bovine Serum, Value, heat inactivated (formerly USDA-approved in North America or qualified, Brazil in other regions), Cat. No. A5256801.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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