E-Gel™ EX Double Comb Agarose Gels
E-Gel™ EX Double Comb Agarose Gels
Invitrogen™

E-Gel™ EX Double Comb Agarose Gels

Invitrogen E-Gel EX Double Comb precast agarose gels are designed for ultrasensitive and convenient DNA sample electrophoresis in as little as 5 to 8 minutes.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberGel PercentageSeparation RangeQuantity
A448881%100 to 5000 bp5 x 10 Gels
A423451%100 to 5000 bp10 Gels
A448871%100 to 5000 bp2 x 10 Gels
A423462%50 to 2000 bp10 Gels
A448892%50 to 2000 bp2 x 10 Gels
A448902%50 to 2000 bp5 x 10 Gels
Catalog number A44888
Price (USD)
1,156.00
Each
Add to cart
Gel Percentage:
1%
Separation Range:
100 to 5000 bp
Quantity:
5 x 10 Gels
Price (USD)
1,156.00
Each
Add to cart
Invitrogen E-Gel EX Double Comb precast agarose gels are designed for ultrasensitive and convenient DNA sample electrophoresis in as little as 5–8 minutes. Each E-Gel EX Double Comb gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel EX Double Comb gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, and genotyping samples.

Features of E-Gel EX Double Comb Agarose Gels include:

  • Instantaneous setup—precast E-Gel agarose gels are ready for use when you are, and do not require gel preparation or additional buffers
  • High sensitivity—detect 1 ng of sample DNA
  • Rapid analysis—complete DNA sample separation in 5–8 minutes
  • In-process control—view DNA sample migration in real time
  • Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
  • For DNA—ideal for DNA sample analysis

Ultra-sensitive detection of DNA

E-Gel EX Double Comb gels include SYBR Gold DNA stain and were developed for ultimate sensitivity, demonstrating up to five-fold greater sensitivity than comparable gels containing ethidium bromide. This superior sensitivity allows you to use lower amounts of sample, saving time and money.

Fast and convenient analysis

E-Gel EX Double Comb gels are our fastest-resolving agarose gels, enabling sample analysis in as little as 5-8 minutes. Each E-Gel EX Double Comb gel contains agarose, electrodes, SYBR Gold DNA stain, and a bufferless ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel staining steps. Just load your samples and run.

Live monitoring of DNA migration

E-Gel EX Double Comb gels include SYBR Gold II DNA gel stain with an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, less-hazardous real-time monitoring of DNA migration is enabled without risk to eyes or possible damage of the sample, unlike UV illumination.

Easy access to the gel

E-Gel EX Double Comb cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), for easy excision of specific bands or transfer of the gel to a membrane for southern blot analysis.

Running and imaging devices

E-Gel EX Double Comb gels require the Invitrogen E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel EX Double Comb gel separation and analysis.

Available in a variety of gel formats

E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 22-well E-Gel EX Double Comb gels are available in 1% and 2% gel percentages.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible LaddersE-Gel™ 1 Kb Plus Express Ladder
Gel Percentage1%
Gel TypeE-Gel
Product TypeAgarose Gel
Separation Range100 to 5000 bp
Stain TypeSYBR™ Gold
Wells22-well
Quantity5 x 10 Gels
Well DesignDouble Comb
Unit SizeEach
Contents & Storage
• Each box contains 10 gels

Store strictly at room temperature. Do not freeze.
Avoid cold spots near freezers or other uncontrolled environments.

Frequently asked questions (FAQs)

I am not seeing any current when I try to run my E-Gel EX agarose gels. Why is this?

Here are some common reasons why your gel is not running properly:

- Copper contacts in the base are damaged due to improper use. Make sure the copper contacts in the base are intact.
- An expired or defective gel cassette was used.
- The E-Gel EX cassette was not inserted properly into a base.
- An incorrect adaptor was used.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

My E-Gel agarose gel has speckles when viewed in the imager. What should I do?

Here are some suggestions:

- Try cleaning the cassettes with alcohol and Kimwipes wipers.
- Try cleaning the camera lens.
- Try to adjust the exposure time and brightness options of the documentation system you are using.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

My sample is leaking from the wells when running my E-Gel agarose gels. What happened?

Please ensure that you have not overloaded the well and that the wells were not damaged during comb removal.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I accidentally stored my E-Gel agarose gels at 4 degrees C instead of room temperature. Can I still use them?

While we recommend storage at room temperature, these gels will still be usable. Bring the gels to room temperature prior to the run for optimal conditions.

Find additional tips, troubleshooting help, and resources within ourNucleic Acid Gel Electrophoresis and Blotting Support Center.

What loading buffer should I use for my E-Gel agarose gels?

Loading buffer is optional. Samples can be loaded directly into the wells if no buffer is used, or you can dilute them with deionized water or TE buffer. If you want to use a loading buffer, please see the recipes below:

E-Gel agarose gels (including EX)
10 mM Tris-HCl, pH 7.5
1 mM EDTA
0.005% bromophenol blue
0.005% xylene cyanol FF
E-Gel CloneWell II and E-Gel SizeSelect II agarose gels
10 mM Tris-HCl, pH 7.5
1 mM EDTA

Alternatively, you can use 10X BlueJuice Gel Loading Buffer or TrackIt Loading Buffer. Dilute this buffer 50- to 200-fold to obtain optimal results with E-Gel agarose gels.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.