Platinum™ Direct PCR Universal Master Mix
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Platinum™ Direct PCR Universal Master Mix
Invitrogen™

Platinum™ Direct PCR Universal Master Mix

Green features
Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.
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Catalog NumberNo. of Reactions
A44647500500 Reactions
A44647100100 Reactions
A446472004 x 500 Reactions
Catalog number A44647500
Price (USD)
588.00
Each
Add to cart
No. of Reactions:
500 Reactions
Request bulk or custom format
Price (USD)
588.00
Each
Add to cart
Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA. It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for superior performance in direct PCR applications.

Features of Platinum Direct PCR Universal Master Mix include:

  • Direct DNA amplification from various samples
  • Universal primer annealing at 60°C
  • Optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets
  • Superior specificity, sensitivity, and yields

Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples. The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments.

Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Specifications
GC-Rich PCR PerformanceHigh
PolymerasePlatinum II Taq Hot-Start DNA Polymerase
Reaction SpeedFast
Green FeaturesLess hazardous, less waste
Product TypeDirect PCR Universal Master Mix
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR, Real Time PCR (qPCR)
Concentration2X
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
Overhang3'-A
Reaction FormatSuperMix or Master Mix
Size (Final Product)8 kb
Unit SizeEach
Contents & Storage
• 2X Platinum Direct PCR Universal Master Mix (5 mL)
• Lysis Buffer (2 x 12.5 mL)
• Proteinase K (0.75 mL)
• Platinum GC Enhancer (2 x 1.25 mL)
• Nuclease-free water (5 mL)

Store at –20°C.

Frequently asked questions (FAQs)

Can I use plant or tissue sample that is not on the list of the Platinum Direct PCR Universal Master Mix tested samples?

You can use any plant or tissue sample. However, when working with new sample materials, we recommend using the Lysis protocol as it allows several PCR reactions to be performed from the same sample during optimization. We also recommend having a positive control with purified sample DNA to ensure that the PCR conditions are optimal. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

When performing Direct PCR with the Platinum Direct PCR Universal Master Mix, do I need to use Proteinase K?

Proteinase K is required when PCR is performed directly from tissue samples using the Direct PCR protocol. Cell debris present in these PCR products can cause DNA fragments to get trapped in the agarose gel wells and Proteinase K helps to eliminate this.

Is the PCR product from reactions performed with Platinum Direct PCR Universal Master Mix compatible with E-Gel agarose gels?

Electrophoretic separation on E-Gel agarose gels depends on the salt concentration in the analyzed sample. For optimal band separation, we recommend diluting PCR reactions 2- to 20-fold with water, prior to running on E-Gel agarose gels. The dyes in the Platinum Direct PCR Universal Master Mix do not interfere with fragment separation on E-Gel agarose gels.

With the Platinum Direct PCR Universal Master Mix, can I leave the samples in the Lysis Solution (Lysis Buffer containing Proteinase K) for longer than 1 min?

Samples can be incubated for up to 8 hr at room temperature in the Lysis Solution, prior to the 98 degrees C Proteinase K inactivation step. Please note that longer incubation may increase the product yield. For longer incubation, the Proteinase K inactivation step may be increased up to 10 min.

What is the biggest sample size that I can use with Platinum Direct PCR Universal Master Mix?

For the Direct protocol, the recommended sample size is up to 1 mm. For the Lysis protocol, the recommended sample size is up to 2 mm. Sample size can be increased up to 1 cm, however, Lysis Buffer volume needs to be increased to cover the whole sample.

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