CTS™ Opti-MEM™ I Medium
CTS™ Opti-MEM™ I Medium
Gibco™

CTS™ Opti-MEM™ I Medium

Gibco CTS Opti-MEM I Medium is an improved minimal essential medium (MEM) that is recommended for use with the CTSRead more
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Catalog NumberQuantity
A4124801100 mL
A4124802500 mL
Catalog number A4124801
Price (USD)
36.91
Each
Add to cart
Quantity:
100 mL
Price (USD)
36.91
Each
Add to cart

Gibco CTS Opti-MEM I Medium is an improved minimal essential medium (MEM) that is recommended for use with the CTS LV-MAX Transfection Kit as a complexation buffer. This medium contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. CTS Opti-MEM I uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. The formulation of CTS Opti-MEM I is identical to research-grade Opti-MEM I Reduced Serum Medium.

CTS Opti-MEM I is xeno-free and contains human transferrin.

CTS Opti-MEM I is manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

Custom configuration and packaging available upon request.

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.
Specifications
Cell LineViral Production Cells
Cell TypeHEK293
ClassificationAnimal Origin-free
FormLiquid
Product TypeMinimum Essential Medium (MEM)
Shelf Life18 months from date of manufacture
SterilitySterile-filtered
FormatBottle
For Use With (Application)LV Production
Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820
Quantity100 mL
Shipping ConditionAmbient
Unit SizeEach
Contents & Storage
100 mL bottle. Store at 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

Why do you recommend using V bottom 96-well plates for harvesting lentivirus from CTS Viral Production Cells?

We recommend using V-bottom 96-well plates to harvest the supernatant containing lentiviral particles as they allow for easier detection of the cell pellets after centrifugation of the cultures.

During lentivirus production from CTS Viral Production Cells, will the shaker vibration affect the cell growth of other cultures maintained in the same incubator or in stacked incubators?

We recommend maintaining shaking cultures in a separate incubator from all other incubators to avoid vibration effects. Alternatively, we recommend using a small, quiet shaker such as the Thermo Scientific CO2 Resistant Shaker (Cat. No. 88881102) for convenience and best performance.

Is 8% CO2 a growth requirement for CTS Viral Production Cells?

We generally recommend culturing the CTS Viral Production Cells under 8% CO2 conditions. We found that cells cultured in 5% CO2 grew slower and were more prone to more cell clumping than when grown in 8% CO2.

What is special about the CTS LV-MAX lentivirus production system?

The CTS LV-MAX lentivirus production system is a fully-optimized complete system in which the cells, media, and transfection reagent work together to generate high-titer lentivirus. The CTS LV-MAX Production Medium is specifically designed to work with the system, to support high density suspension cell growth of CTS Viral Production Cells.

The manual recommends using CTS Viral Production Cells passaged to P5 (˜3 weeks) on the day of transfection. Are these general cell culture guidelines or special requirements for these cells?

Although 3-4 passages before transfection are sufficient for most cell lines recovering directly from liquid N2 storage, we found that CTS Virus Production Cells passaged to P5 showed the highest performance in lentivirus particle production and reproducibility between batches. Therefore, we recommend passaging the cells to P4 at the end of a given week for lentivirus production the following week.

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