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Expi293™ Inducible GnTI- Expression System Kit
The Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression fromRead more
Catalog number A39252
Price (USD)
3,930.00
Each
Price (USD)
3,930.00
Each
The Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The Expi293F Inducible GnTI- cell line was established by the selective knockout of the GnTI gene and the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter, in parental Expi293F cells. This cell line is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.
Expi293 Inducible GnTI- Expression System Kit features include:
• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers
The components included in the Expi293 Inducible GnTI- Expression System Kit are: one vial of frozen Expi293F Inducible GnTI- cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one PNGase F Glycan Cleavage kit, one pcDNA5/TO Mammalian Expression Vector kit, 500 mg tetracycline hydrochloride, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible GnTI- Expression System are also available for individual purchase.
Expi293 Inducible GnTI- Expression System Kit features include:
• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers
The components included in the Expi293 Inducible GnTI- Expression System Kit are: one vial of frozen Expi293F Inducible GnTI- cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one PNGase F Glycan Cleavage kit, one pcDNA5/TO Mammalian Expression Vector kit, 500 mg tetracycline hydrochloride, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible GnTI- Expression System are also available for individual purchase.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Delivery TypeTransfection
Quantity1 kit
Shipping ConditionApproved for shipment at Room Temperature or on Wet or Dry Ice
Cell LineExpi293F™
Product LineExpi293™
For Use With (Application)Protein Production
PromoterCMV/TO
Unit SizeEach
Contents & Storage
• Expi293F Inducible GnTI- Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen
• 1 L Expi293 Expression Medium, store at 2–8°C (protect from light)
• ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C
• PNGase F Glycan Cleavage Kit, store at -20C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• 20 μg pcDNA5/TO Mammalian Expression Vector, store at 2–8°C or long term storage at -20°C.
• 500 mg Tetracycline Hydrochloride, store at -20°C
• pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C
• 1 L Expi293 Expression Medium, store at 2–8°C (protect from light)
• ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C
• PNGase F Glycan Cleavage Kit, store at -20C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• 20 μg pcDNA5/TO Mammalian Expression Vector, store at 2–8°C or long term storage at -20°C.
• 500 mg Tetracycline Hydrochloride, store at -20°C
• pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C