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Gibco™
Poly-D-Lysine
Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-LysineRead more
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Catalog number A3890401
Price (USD)
142.00
Each
Add to cart
Price (USD)
142.00
Each
Add to cart
Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight of 50,000–150,000 Daltons, which makes this product ideal for neuronal culture applications. This ready-to-use product is provided as a liquid at a concentration of 0.1 mg/mL. When used on Nunc cell culture plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.
Key features include: • Synthetic and animal origin-free • Sterile-filtered for cell culture applications • Validated with B-27 Supplement and B-27 Plus Neuronal Culture System when used with Nunc plastic and glass bottom dishes
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ClassificationAnimal Origin-free
Concentration0.1 mg/mL
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeExtracellular Matrix
Shelf Life12 Months
SterilitySterile
Product LineGibco™
Purity or Quality GradeCell Culture Grade
Quantity100 mL
Shipping ConditionApproved for shipment on Wet Ice
SourceAnimal origin-free, Synthetic
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).
Frequently asked questions (FAQs)
What solution is Poly-D-Lysine (Cat. No. A3890401) delivered in?
Yes, Poly-D-Lysine can undergo one freeze-thaw cycle without impacting performance. Please note that this does not extend the shelf-life of the product.
I coated my plates with Poly-D-Lysine and I'm noticing that the cells are dying within a few days of plating the cells. How can I ensure cell survival?
It is possible that the plates were not adequately rinsed with water prior to plating the cells. Residual Poly-D-Lysine is toxic and may be the cause of cell death. It is recommended to rinse the plates with water three times before exposing the cells to the matrix.
I coated my plates with Poly-D-Lysine and I'm noticing that the cells are not attaching well. What can I do to prevent the cells from lifting off?
When coating the surface with Poly-D-Lysine, make sure that the entire surface is fully covered and that the appropriate volume of coating matrix is applied.