Poly-D-Lysine
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Poly-D-Lysine
Gibco™

Poly-D-Lysine

Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-LysineRead more
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Catalog number A3890401
Price (USD)
142.00
Each
Add to cart
Price (USD)
142.00
Each
Add to cart
Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight of 50,000–150,000 Daltons, which makes this product ideal for neuronal culture applications. This ready-to-use product is provided as a liquid at a concentration of 0.1 mg/mL. When used on Nunc cell culture plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.

Key features include:
• Synthetic and animal origin-free
• Sterile-filtered for cell culture applications
• Validated with B-27 Supplement and B-27 Plus Neuronal Culture System when used with Nunc plastic and glass bottom dishes

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ClassificationAnimal Origin-free
Concentration0.1 mg/mL
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeExtracellular Matrix
Shelf Life12 Months
SterilitySterile
Product LineGibco™
Purity or Quality GradeCell Culture Grade
Quantity100 mL
Shipping ConditionApproved for shipment on Wet Ice
SourceAnimal origin-free, Synthetic
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

What solution is Poly-D-Lysine (Cat. No. A3890401) delivered in?

Poly-D-Lysine is in PBS solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I freeze Poly-D-Lysine?

Yes, Poly-D-Lysine can undergo one freeze-thaw cycle without impacting performance. Please note that this does not extend the shelf-life of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are there any animal origin components in Poly-D-Lysine?

The Poly-D-Lysine matrix is animal origin-free.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I coated my plates with Poly-D-Lysine and I'm noticing that the cells are dying within a few days of plating the cells. How can I ensure cell survival?

It is possible that the plates were not adequately rinsed with water prior to plating the cells. Residual Poly-D-Lysine is toxic and may be the cause of cell death. It is recommended to rinse the plates with water three times before exposing the cells to the matrix.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I coated my plates with Poly-D-Lysine and I'm noticing that the cells are not attaching well. What can I do to prevent the cells from lifting off?

When coating the surface with Poly-D-Lysine, make sure that the entire surface is fully covered and that the appropriate volume of coating matrix is applied.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.