The Thermo Scientific Pro-Detect Rapid V5 Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detectionRead more
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Catalog number A38513
Price (USD)
134.00
Each
Add to cart
Price (USD)
134.00
Each
Add to cart
The Thermo Scientific Pro-Detect Rapid V5 Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of V5-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to V5 tag to provide a qualitative detection within ten minutes. This assay can be used to detect V5-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.
Features of the Pro-Detect Rapid competitive assay kits: • Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target • Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes • Sensitive—detect protein tags in supernatant or cell lysate samples down to low μg/mL concentrations • Specific—assay does not cross-react with common lysis and protein preparation reagents • Reliable—results are similar to other antibody immunoassays, including western blot and ELISA • Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detectable AnalytesV5-tagged proteins
AssayLateral Flow Rapid Assay
Detection MethodChromogenic
Product LinePro-Detect™
Product TypeAssay
SpecificityProtein tag
Sufficient For10 Assays
For Use With (Application)Lateral Flow Rapid Assay