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TrueCut™ Cas9 Protein v2
Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency. Features include:• ConsistentlyRead more
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| Catalog Number | Concentration | Components |
|---|---|---|
| A36497 | 1 μg/μL | 25μL TrueCut Cas9 Protein v2 (1 mg/mL) |
| A36498 | 5 μg/μL | 20μL TrueCut Cas9 Protein v2 (5 mg/mL) |
| A36496 | 1 μg/μL | 10μL TrueCut Cas9 Protein v2 (1 mg/mL) |
| A36499 | 5 μg/μL | 100μL TrueCut Cas9 Protein v2 (5mg/mL) |
Catalog number A36497
Price (USD)
105.00
Each
Concentration:
1 μg/μL
Components:
25μL TrueCut Cas9 Protein v2 (1 mg/mL)
Price (USD)
105.00
Each
Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency. Features include:
• Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
• High quality—manufactured under strict ISO 13485 quality standards
• Validated protocols for a large number of cell types help you achieve success faster
TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.
• Transfection-ready, using either lipid-mediated transfection reagents or electroporation
• Eliminates time-consuming cloning steps
• Available in 2 concentrations:
--1 μg/μL for use in standard editing scenarios
--5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
--For custom sizes or concentrations, please inquire
Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:
1. Order transfection-ready TrueGuide synthetic gRNAs
2. Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit
3. Have our Custom Services team design, synthesize, and purify in vitro (IVT) gRNA sequences for you
• Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
• High quality—manufactured under strict ISO 13485 quality standards
• Validated protocols for a large number of cell types help you achieve success faster
TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.
• Transfection-ready, using either lipid-mediated transfection reagents or electroporation
• Eliminates time-consuming cloning steps
• Available in 2 concentrations:
--1 μg/μL for use in standard editing scenarios
--5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
--For custom sizes or concentrations, please inquire
Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:
1. Order transfection-ready TrueGuide synthetic gRNAs
2. Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit
3. Have our Custom Services team design, synthesize, and purify in vitro (IVT) gRNA sequences for you
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 μg/μL
Components25μL TrueCut Cas9 Protein v2 (1 mg/mL)
GradeMolecular Biology
Accession NumberNC_002737.2:854751-858857
Expression SystemE. coli
Gene ID (Entrez)901176
Gene SymbolSpy_1046
Gene Aliascas5, csn1
Protein FormRecombinant
Protein TagUntagged
Shipping ConditionDry Ice
Validated ApplicationGenome Editing
Product TypeCas9 Protein v2
Research CategoryGenome Editing
Product LineInvitrogen™
Unit SizeEach
Contents & Storage
25 μL TrueCut Cas9 Protein v2 (1 mg/mL)
Store at -5 to -30°C.
Store at -5 to -30°C.