High Select™ Depletion Spin Columns
High Select™ Depletion Spin Columns
High Select™ Depletion Spin Columns
High Select™ Depletion Spin Columns
Thermo Scientific™

High Select™ Depletion Spin Columns

Decrease albumin, immunoglobulins, and other high-abundance proteins from human serum or plasma in preparation for mass spectrometry analysis, or 1D and 2D gel electrophoresis using High Select depletion spin columns or resin.
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Catalog NumberDescription
A36369Top14 Abundant Protein Depletion Mini Spin Columns
A36365HSA/Immunoglobulin Depletion Mini Spin Columns
A36366HSA/Immunoglobulin Depletion Mini Spin Columns
A36367HSA/Immunoglobulin Depletion Midi Spin Columns
A36368HSA/Immunoglobulin Depletion Resin
A36371Top14 Abundant Protein Depletion Midi Spin Columns
A36372Top14 Abundant Protein Depletion Resin
A36370Top14 Abundant Protein Depletion Mini Spin Columns
Catalog number A36369
Price (USD)
340.00
Each
Add to cart
Description:
Top14 Abundant Protein Depletion Mini Spin Columns
Price (USD)
340.00
Each
Add to cart
Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein. Removal of albumin, immunoglobulins, and other abundant proteins is essential for the study of low-abundance proteins. Thermo Scientific High Select HSA/Immunoglobulin and Top 14 Abundant Protein depletion spin columns and resins enable depletion of the most abundant proteins in human serum and plasma for improved detection of low-abundance proteins using mass spectrometry analysis or 1D or 2D gel electrophoresis.
Flexible—resin provided in mini and midi column formats as well as bulk for custom filling options
Optimized—removes >95% of IgG and >95% of albumin (HSA/Immunoglobulin); removes >95% of IgG and >95% of albumin, plus 12 other abundant proteins (Top 14)
Fast—process samples in ∼10 minutes
Economical—cost-effective spin columns are priced for single use
Reduced carry-over—one-time use prevents protein carryover and experimental variability

High Select HSA/Immunoglobulin Depletion Resin uses highly specific, immobilized anti-HSA and anti-immuoglobulin antibodies (IgG, IgA, IgM, IgD, and IgE) to remove human serum albumin (HSA) and all major subclasses of immunoglobulins from serum and plasma. High Select Top14 Abundant Protein Depletion Resin uses highly specific immobilized antibodies to remove human serum albumin (HSA), albumin, IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, alpha-1-acidglycoprotein, alpha-1-antitrypsin, alpha-2-macroglobulin, apolipoprotein A1, fibrinogen, haptoglobin, and transferrin from serum, plasma, or spinal fluids. The resins are provided in an economical and convenient spin column format specifically designed for one-step processing and single use or bulk format for custom filling.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionTop14 Abundant Protein Depletion Mini Spin Columns
For Use With (Equipment)Mass Spectrometer
Product TypeColumn
Product LineHigh Select™
Label or DyeUnlabeled
Shipping ConditionWet Ice
Starting MaterialPlasma, Serum
Final Product TypeProtein
FormatMini Spin Column
Workflow StepProtein Depletion
Quantity6 Columns
Unit SizeEach
Contents & Storage
Store at 2–8°C.

Frequently asked questions (FAQs)

Can I use the High Select Top14 Abundant Protein Depletion Midi Spin Columns to deplete proteins from rodent samples?

The High Select Top14 Abundant Protein Depletion Midi Spin Columns have only been validated for human samples. We do not recommend using them for protein depletion in rodent samples. For protein depletion in rodent samples, we recommend the following multi-species depletion resins:

  • CaptureSelect MultiSpecies Albumin Depletion Product (Cat. No. 191085305)
  • Pierce Albumin Serum Depletion Kits (Cat. No. 89875)


  • Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are your High Select Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) compatible with cell culture media?

Our High Select Top14 Abundant Protein Depletion Mini Spin Columns have been tested and validated for protein depletion only on human plasma and serum, with each column optimized to bind up to 10 µL of sample.
We have not tested our product on cell culture media or with serum from different species, therefore we do not recommend using this product with cell culture media.

Is there a 96-well format available for the Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369)?

No. The Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) do not come in a 96-well format. However, we do have a Top14 Abundant Protein Depletion Resin (Cat. No. A36372) that can be purchased as a stand-alone product.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After depletion of abundant proteins from my sample, I still see albumin and other abundant proteins after LC-MS analysis. Did the depletion work?

Depletion does not remove all abundant proteins from the sample. Even with 99% depletion of a specific plasma protein, highly abundant proteins will still be the most abundant proteins in the sample detected by MS analysis. However, depletion significantly decreases the most abundant plasma proteins enabling identification of lower abundant proteins.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I proceed to downstream mass spec sample preparation after abundant plasma protein depletion?

After the depletion, samples are significantly diluted in buffer. Depleted samples should be concentrated by speed vac drying, solvent precipitation, or diafiltration. Samples dried using a speedvac are directly compatible with the EasyPep kit chemistry after resuspension using the Lysis Solution.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.