Neurobasal™ Plus Medium
Culturing neurons? Try our new Gibco™ Poly-D-Lysine Solution.
Neurobasal™ Plus Medium
Gibco™

Neurobasal™ Plus Medium

Neurobasal Plus Medium is the next-generation of one of the most cited neuronal cell culture products, Neurobasal Medium. It isRead more
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Catalog number A3582901
Price (USD)
127.00
Each
Add to cart
Price (USD)
127.00
Each
Add to cart
Neurobasal Plus Medium is the next-generation of one of the most cited neuronal cell culture products, Neurobasal Medium. It is a neuronal basal medium that provides the highest rates of in vitro survival of primary rodent and human stem cell-derived neurons when used with B-27 Plus Supplement. Neurobasal Plus Medium features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rates of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.

Neurobasal Plus Medium, when combined with B-27 Plus Supplement, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic Neurobasal Medium and B-27 Supplement
• Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
• Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Available as a 500 mL basal medium, Neurobasal Plus Medium can seamlessly replace other neuronal cell culture media, such as classic Neurobasal Medium. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Cells
ClassificationSerum-free
Culture TypeNeuronal Cell Culture
FormLiquid
Product TypeNeurobasal Medium
Shelf Life12 Months From Date of Manufacture
With AdditivesPhenol Red
Product LineNeurobasal™
Quantity500 mL
Shipping ConditionAmbient
Tested ForEndotoxin, Performance, Sterility, Osmolality, pH
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?

We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the osmolality of Neurobasal Plus Medium?

The osmolality is in the range of 215-235 mOsm/kg. The exact osmolality for your lot of the medium can be found in the Certificate of Analysis for that lot number.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.