LV-MAX™ Transfection Kit
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LV-MAX™ Transfection Kit
Gibco™

LV-MAX™ Transfection Kit

The Gibco LV- MAX Transfection Kit is an essential component of the LV-MAX Lentiviral Production System. It is formulated forRead more
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Catalog number A35348
Price (USD)
1,650.00
Each
Add to cart
Price (USD)
1,650.00
Each
Add to cart
The Gibco LV- MAX Transfection Kit is an essential component of the LV-MAX Lentiviral Production System. It is formulated for the transient transfection of Gibco Viral Production Cells (high-density suspension-adapted human embryonic kidney (HEK) 293-derived cells). The included high-efficiency, cationic, lipid-based transfection reagent, lentiviral production supplement, and LV enhancer are designed to work together to produce the highest possible level of lentiviral particles from the Viral Production Cells when cultured in LV-MAX Production Medium.

• Designed specifically for transfection of high-density suspension cells to maximize lentiviral production
• Enables sustained, high-level production of high-density transiently-transfected cultures, achieving titer of up to 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Reproducible and scalable transfection results, giving users greater confidence in their results
• Easy, robust culture and transfection protocols—fits into any transient production workflowsThe kit includes three key components required to optimize your lentiviral production. These are only available in the kit and are not sold separately.
• LV-MAX transfection reagent—a high-efficiency, cationic, lipid-based transfection reagent uniquely designed for co-transfecting multiple plasmids into high-density Viral Production Cell cultures with high transfection efficiency and low toxicity
• LV-MAX supplement—an optimized, chemically defined, serum-free, protein-free, animal origin-free formulation designed to suppress cell growth during transfection and increase lentiviral vector production without compromising the cell viability and interfering with the lentiviral production process
• LV-MAX enhancer—a proprietary, animal origin-free formulation designed to boost cell lentiviral vector production

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineEstablished Cell Lines
ClassificationAnimal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Cell Type293-derived Cells
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Product LineLV-MAX™
Product TypeTransfection Kit
Quantity1 L
Serum CompatibleYes
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
• 50 mL LV-MAX Supplement
• 6 mL LV-MAX Transfection Reagent
• 40 mL LV-MAX Enhancer

Sufficient for 1 L LV production 

Store at 2–8°C, protect from light.

Frequently asked questions (FAQs)

What are the major differences between LV-MAX and CTS LV- MAX products?

The LV-MAX and CTS LV- MAX products have equivalent performance. In contrast to the research use only LV-MAX products, CTS LV-MAX products have an additional intended use for cell and gene therapy applications, and are complemented with a more robust regulatory support package to enable clinical therapeutic applications. This means that to the best of our ability, we keep our CTS product specifications and release requirements up-to-date in accordance with the latest regulatory guidance specific for this intended use.

What is the intended use claim for your LV-MAX and CTS LV-MAX products?

Our LV-MAX products are intended for research use only while our CTS LV- MAX products are intended for research use or manufacture of cell, gene or tissue based products. Please refer to the individual products for further details on the intended use claim.

Do you offer a lentivirus production service?

Yes. Please provide your details here (https://www.thermofisher.com/us/en/home/global/forms/life-science/lentiviral-vector-production-get-info.html) and mention your interest in our lentivirus production services. A member of our services team will contact you shortly.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

With the LV-MAX Production System, are the lentiviral particles stable in packaging media?

Lentiviral particles packaged in our LV-MAX Production Medium will be stable at 4 degrees C for 24 hours and will then begin to lose activity. For long-term storage, we recommend storing at –80 degrees C.

With the LV-MAX Production System, can I use cell lines besides HT1080 for titering my lentivirus?

We strongly recommend the human fibrosarcoma line HT1080 (ATCC, Cat. No. CCL-121) as the “gold standard” for reproducibly titering lentivirus. However, you may wish to use the same mammalian cell line to titer your lentiviral stocks as you will use to perform your expression studies (e.g., if you are performing expression studies in a dividing cell line or a non-primary cell line). If you have more than one lentiviral construct, we recommend that you titer all of the lentiviral constructs using the same mammalian cell line.