StemFlex™ Medium
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StemFlex™ Medium
Gibco™

StemFlex™ Medium

Gibco™ StemFlex™ Medium supports the robust expansion of feeder-free pluripotent stem cells (PSCs) and is optimized to support novel applications,Read more
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Catalog number A3349401
Price (USD)
403.00
Each
Add to cart
Price (USD)
403.00
Each
Add to cart
Gibco™ StemFlex™ Medium supports the robust expansion of feeder-free pluripotent stem cells (PSCs) and is optimized to support novel applications, including single-cell passaging, gene editing, and reprogramming. StemFlex Medium’s unique formulation offers the convenience of a flexible feeding schedule (including weekend-free options) and also the ability to choose the matrix and passaging reagent that best suits specific applications. StemFlex Medium enables the long term feeder-free culture of PSCs without karyotypic abnormalities and maintains the cells' ability to differentiate into all three germ layers.

Superior results
StemFlex Medium is formulated to deliver superior outcomes in today’s more-challenging applications that stress PSC cultures, such as reprogramming, single cell passaging, and gene editing. StemFlex Medium provides superior cell survival compared to the leading competitor (mTeSR1), allowing for greater success in the most relevant cell types.

Flexibility
StemFlex Medium allows for unprecedented 3-way usage flexibility for PSC culture conditions: the ability to select an optimal feeding schedule, including a weekend-free option; a choice of matrices, depending upon the needs of the workflow; and the option to select the passaging reagent for the level of cell dissociation required.

Exceptional value and performance
StemFlex Medium is provided in a convenient two-component kit (450 mL basal medium & 50 mL supplement), and when used with Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix, provides a cost-effective, robust system for superior feeder-free culture of human PSCs. StemFlex Medium’s ability to enable weekend-free culture of PSCs provides unmatched value among the robust feeder-fee PSC media on the market today.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEmbryonic Stem Cells, , Induced Pluripotent Stem Cells
Culture TypeFeeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic)
Product TypeStem Cell Media
With AdditivesPhenol Red
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product LineStemFlex™
Quantity500 mL
Shipping ConditionSupplement ships on dry ice, basal can ship at RT
Unit SizeEach
Contents & Storage
• 450 mL Basal Medium, store at 2–8°C and protect from light
• 50 mL Supplement, store at -5 to -20°C and protect from light

Frequently asked questions (FAQs)

What is the glucose content of the StemFlex Medium (Cat. No. A3349401)?

The StemFlex Medium (Cat. No. A3349401) includes 3.151 g/L (~17.5 mM) of D-glucose (dextrose) in the Basal Medium. The StemFlex Medium Supplement does not include glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What media do you recommend for standard single-cell cloning of iPSCs?

We recommend StemFlex Medium (Cat. No. A3349401) or Essential 8 (Cat. No. A1517001) for standard single-cell iPSC cloning in the presence of RevitaCell or ROCK inhibitor. StemScale PSC Suspension Medium (Cat. No. A4965001) is not recommended for iPSC cloning.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does StemFlex Medium contain pen/strep antibiotic?

StemFlex Medium does not contain pen/strep antibiotic.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between StemFlex Medium and Essential 8 Flex Medium?

Essential 8 Flex Medium is a serum-free, xeno-free medium that contains proprietary material to extend the activity of the key heat-sensitive component, FGF2. Therefore, it supports the culture and expansion of feeder-free pluripotent stem cells (PSCs) without the need for daily or weekend feeding. Note: We also offer Essential 8 Medium that does not allow skipping of PSC feeding during the weekend.

StemFlex Medium also supports the expansion of feeder-free PSCs with a flexible feeding schedule (including weekend-free feeding) and also the ability to choose the matrix and passaging reagent that best suits specific applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Has StemFlex Medium been evaluated for growth of mouse ESCs?

StemFlex Medium contains basic FGF which has been shown to result in differentiation of mouse embryonic stem cells (mESCs) to mouse-derived epiblast stem cells (mEpiSCs). Also, mESCs demonstrate dependence on leukemia inhibitor factor (LIF). Therefore, StemFlex Medium is not recommended for culture of mESCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.