Fetal Bovine Serum, certified, United States
Fetal Bovine Serum, certified, United States
Gibco™

Fetal Bovine Serum, certified, United States

Green features
Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust ofRead more
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Catalog NumberQuantityFormat
A316040150 mLOne Shot
A316040210 x 50 mLOne Shot
16000044500 mLBottle
160000691000 mLBottle
Catalog number A3160401
Price (USD)
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Quantity:
50 mL
Format:
One Shot

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Gibco Sera: Trusted Quality for Consistent Performance

Sera Category: Premium (Performance Plus)

  • Use for a broad range of cell types, especially sensitive cell lines
  • Our most characterized sera with the lowest endotoxin and hemoglobin levels
  • Endotoxin level: ≤5 EU/mL
  • Hemoglobin level: ≤15 mg/dL
  • Origin: United States

Gibco Serum Delivers

  • ISO 13485 certified, processed in FDA registered facilities
  • Triple filtered at 0.1 μm
  • Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
DescriptionFetal Bovine Serum, certified, One Shot™ format, United States
AgeFetal
SpeciesBovine
Country of OriginUnited States
FormLiquid
FormatOne Shot
Product TypeFetal Bovine Serum
Quantity50 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Sterilization MethodTriple-filtered, 0.1 μm
Endotoxin Concentration≤5 EU/mL
Green FeaturesFewer resources used and less waste
Hemoglobin Concentration≤15 mg/dL
Purity or Quality GradeCertified
Product LineOne Shot™
Shipping ConditionFrozen
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.