7500 Fast Food Safety Real-Time PCR System, laptop
7500 Fast Food Safety Real-Time PCR System, laptop
Applied Biosystems™

7500 Fast Food Safety Real-Time PCR System, laptop

Meet your pathogen and authenticity testing needs with the Applied Biosystems 7500 Fast Food Safety Real-Time PCR System. This isRead more
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Catalog NumberDescription
A302997500Fast w/ Laptop
Catalog number A30299
Price (USD)
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Description:
7500Fast w/ Laptop
Meet your pathogen and authenticity testing needs with the Applied Biosystems 7500 Fast Food Safety Real-Time PCR System. This is an integrated and versatile real-time PCR system that combines thermal cycling, fluorescence detection, and application-specific software to measure the cycle-by-cycle accumulation of PCR products in a single-tube homogeneous reaction.

The 7500 Fast Food Safety Real-Time PCR System is compatible with all Thermo Fisher Scientific food safety PCR assays. These assays offer detection of the major pathogens and other important targets in food samples.

Expand your in-house PCR capability beyond day-to-day pathogen testing with the 7500 Fast Real-Time PCR System—a versatile, 96-well platform that provides flexibility to tackle your most challenging test requirements.

Flexible—five-color detection system allows a variety of applications including gene expression analysis, SNP genotyping, and plus/minus assays that utilize internal positive controls
Efficient—facilitates processing of multiple assays in the same run
Convenient 96-well format—minimizes the effort required to perform sample setup and allows the use of tube strips
Simple intuitive software—enables rapid, straightforward PCR set-up, automatic data interpretation, and traceability of results
Small instrument footprint—fits easily into laboratory environments where bench space is at a premium
Instrument service and support—instruments are fully supported by our own service engineers and dedicated, knowledgeable technical team

The 7500 Fast Real-time PCR System is compatible with our SureTect Real-Time PCR Food Pathogen Assays; MicroSEQ, RapidFinder, and TaqMan assays; and RapidFinder Salmonella species, Typhimurium, and Enteritidis Multiplex Assay.

The 7500 Fast Real-Time PCR System delivers high-speed cycling capabilities with a new fast-ramping Peltier-based thermal cycling block. The instrument, Optical Fast 96-well reaction plates, and hot-start DNA polymerase containing master mix comprise the complete Fast system. With this system, quantitative real-time PCR runs can be completed in about 50 minutes with comparable performance to standard run times for supported applications.

The advanced optical configuration supports a broader range of fluorophores, including FAM/SYBR Green I, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 dyes; variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

For Testing of Food and Environmental Samples Only.
For Testing of Food and Environmental Samples Only.
Specifications
Format96-well Plate
Thermal Accuracy±0.0.25°C (35°C to 99.9°C)
High-throughput CompatibilityHigh-throughput Compatible
For Use With (Equipment)7500 Fast System
Thermal Uniformity<0.5 °C (20 s after reaching 95°C)
Reaction SpeedFast
Capacity12 x 8-tube Strips, 1 x 96-well Plate (Fast)
Voltage100 to 240 V
Display Type8.4 in. Color TFT LCD
Product TypeThermal Cycler
Height (English)10.6 in.
Depth (English)22 in.
Height (Metric)27.2 cm
Width (Metric)33 cm
Width (English)13 in.
Depth (Metric)56.5 cm
Thermal Range0°C to 100°C
For Use With (Application)Real Time PCR (qPCR)
Shipping ConditionRoom Temperature
User InterfaceEthernet
Max. Ramp Rate3°C/s (Block), 1.6°C/s (Sample)
Description7500Fast w/ Laptop
Weight20.4 kg (45 lb.)
Block Configurations1 x 96-well Plate
Unit SizeEach
Package Components
7500 Fast Real-Time PCR System, laptop, QST
Catalog Number
4365464
Unit Size
1 system
Qty
1
7500 Fast Precision Plate Holder, for 0.1 mL tube strips
Catalog Number
A29252
Unit Size
1 each
Qty
1
RapidFinder™ Express Software v2.0 CD Kit
Catalog Number
A28811
Unit Size
1 kit
Qty
1
MicroAmp™ Cap Installing Tool
Catalog Number
4330015
Unit Size
1 tool
Qty
1
MicroAmp™ Optical 8-Cap Strips
Catalog Number
4323032
Unit Size
300 strips
Qty
1
MicroAmp™ Fast 8-Tube Strip, 0.1 mL
Catalog Number
4358293
Unit Size
125 strips
Qty
1
Contents & Storage
Includes One Real Time PCR instrument, one Dell laptop computer, RapidFinder Express v2.0 Software, and RNase plate.

Frequently asked questions (FAQs)

My 7500 Fast Real-Time PCR System has been upgraded to v2.0.6, so why can't I analyze my files with HRM Software v2.0.1?

For the 7500 Fast Real-Time PCR System, if the software version is below v2.0.4, the compatible HRM Software is v2.0.1. If the 7500 Fast Real-Time PCR System has been upgraded to software version 2.0.4 or above, then you will need to get HRM Software version 3.0.1.

How do I perform the HRM calibration on my real-time PCR instrument?

The HRM calibration is a 3-step procedure. In some instrument software, the calibration process has been streamlined to a single-step procedure (applies to the ViiA7 Real-Time PCR System and QuantStudio Real-Time PCR Systems).

For the HRM calibration, the first step is a PCR reaction to generate the HRM dye plate. The reaction plate contains a DNA template, a primer pair, and the PCR mix with HRM dye. The second step is dye calibration using the PCR plate from step 1. The last step is a melt curve run, again using the same plate. For details, please refer to the HRM guides for each instrument:

- 7500 Fast Real-Time PCR System (with software version 1.4 or 2.0.4 and below) (https://tools.thermofisher.com/content/sfs/manuals/cms_050347.pdf)
- 7500 Fast Real-Time PCR System (with software version 2.0.5 and above) (https://tools.thermofisher.com/content/sfs/manuals/4457847.pdf)
- 7900HT Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/cms_050347.pdf)
- StepOne and StepOnePlus Real-Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/4457847.pdf)
- ViiA 7 Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/4443531.pdf)
- QuantStudio 6 and 7 Real-Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/4489823.pdf)
- QuantStudio 6 and 7 Pro Real-Time PCR Systems (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018981_HighResolutionMelt_DA_SW_UG.pdf)
- QuantStudio 3 and 5 Real Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/MAN0014394_HighResMeltExperiment_GSG.pdf)
- QuantStudio 12K Flex Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/4470692A.pdf)

Find additional tips, troubleshooting help, and resources within our High Resolution Melt Support Center.

Is my real-time PCR instrument compatible with HRM?

Among our real-time PCR instruments, the following are compatible with HRM:

- 7500 Fast Real-Time PCR System
- 7900HT Fast Real-Time PCR System
- StepOne and StepOnePlus Real-Time PCR Systems
- ViiA 7 Real-Time PCR System
- QuantStudio 6 and 7 Pro Real-Time PCR Systems
- QuantStudio 6 and 7 Flex Real-Time PCR Systems
- QuantStudio 12K Flex Real-Time PCR System
- QuantStudio 1 Real-Time PCR System
- QuantStudio 3 Real-Time PCR System
- QuantStudio 5 Real-Time PCR System

Why are there no RQ values for all the samples in my study from the 7500 or 7500 Fast Real-Time PCR System software?

The 7500 software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have CT values for all the wells, but the software will not calculate RQ values.

If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist software instead. This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7500 software as a *.txt or *.csv file, and use that to create a new study in the DataAssist software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why do I not see any CT or RQ values from my ddCT experiment in the software for the 7500 or 7500 Fast Real-Time PCR System?

If you have the SDS v1.4 or earlier, you will need to open the dd CT plate in a Study first in order to see the CT or RQ values. (If you have v2.0.x this error does not apply.)

1.After the initial run is complete, open, review the plots and save it. Then:
-Choose File then New
-Choose: Assay then ddCT (Relative Quantitation) Study. Set the other parameters as appropriate and click Next.
2.Click on ‘Add Plates...', then browse to where your data file is located and select the plate (or multiple plates).
Click ‘Finish', and you should see your study.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.