Alexa Fluor™ Plus 647 Phalloidin
Alexa Fluor™ Plus 647 Phalloidin
Invitrogen™

Alexa Fluor™ Plus 647 Phalloidin

Alexa Fluor Plus 647 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, Cy5/deep redRead more
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Catalog number A30107
Price (USD)
664.00
Each
Add to cart
Price (USD)
664.00
Each
Add to cart
Alexa Fluor Plus 647 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, Cy5/deep red fluorescent Alexa Fluor Plus 647 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 647 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 647 Phalloidin per length of F-actin.


• Selectively stains F-actin with 2–3 times the sensitivity of Alexa Fluor 647 Phalloidin
• Outstanding fluorescence performance for sharp actin cytoskeleton images
• Excitation/emission: 650/668 nm. Compatible with standard Cy5/deep red/far red filter set or laser settings
• More sensitive and specific than antibody staining against actin
• Optimal for fixed and permeabilized samples

Enables superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides 'death cap' mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 647 dye provides Cy5/far red fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 647 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Dye TypeAlexa Fluor Plus 647
Quantity1 each
Product LineAlexa Fluor™
Shipping ConditionQualified for Ambient or Wet Ice
Label TypeAlexa Fluor Dyes
Product TypePhalloidin
SubCellular LocalizationActin
Unit SizeEach
Contents & Storage
Contains 1 vial with enough material for 300 units/slides
Store at ≤-20°C after receiving. Desiccate and protect from light.
When stored as directed, product is stable for (at least 1 year.
)

Frequently asked questions (FAQs)

Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am labeling cultured cells with phalloidin and not seeing any labeling. What could cause this?

If the F-actin is depolymerized, phalloidin will not bind. Any processing steps or any treatments/compound that can promote depolymerization would impact phalloidin binding. Also, any compounds that also bind to F-actin (without depolymerization) may affect phalloidin binding.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there any difference between the Alexa Fluor phalloidin products and the Alexa Fluor Plus phalloidin products?

No. They differ only in the dyes attached to the phalloidin. The phalloidin has the same structure in all of the products.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.