IonCode™ Barcode Adapters 1-384 Kit

The IonCode Barcode Adapters 1-384 Kit provides a set of 384 unique barcode adapters specifically designed for optimal performance with the Ion GeneStudio S5, Ion PGM, and Ion Proton semiconductor sequencers. When used in combination with Ion Barcode Adapters 385-768 (Cat. No. A36546) and Ion AmpliSeq Library Kit PLUS or Ion AmpliSeq Library Kit 2.0, this kit enables users to pool up to 768 amplicon libraries and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment.

Features of the IonCode Barcode Adapters 1-384 Kit include:

• Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Multiplexing with barcode adapters enables higher throughput

Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 768 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias.

Sequence- and flow-optimized for increased performance and efficiency

Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool and require minimal number of flows to interrogate, resulting in more economical multiplexed sequencing runs.

Robust error correction built in by design

Built-in error correction prevents the possibility of incorrect sample assignment.