The Gibco™ PSC Cardiomyocyte Differentiation Kit consists of a set of serum-free and xeno-free media that enable efficient differentiation ofRead more
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Catalog number A2921201
Price (USD)
742.00
Each
Add to cart
Price (USD)
742.00
Each
Add to cart
The Gibco™ PSC Cardiomyocyte Differentiation Kit consists of a set of serum-free and xeno-free media that enable efficient differentiation of human pluripotent stem cells (PSCs) to contracting cardiomyocytes in as few as 8 days. Unlike other methods that require multiple components and longer assay duration, the PSC Cardiomyocyte Differentiation Kit can generate cardiomyocytes from pluripotent stem cells in a ready-to-use media format and in less time.
Comprised of three 1X media that require no thawing or mixing, each medium is used consecutively over a total of 14 days, resulting in functional cardiomyocytes that express relevant physiological markers, contract in culture, and can be subsequently maintained in culture for >15 days (maintenance medium also sold separately (Cat. No. A2920801)).
No thawing or mixing required The PSC Cardiomyocyte Differentiation Kit is comprised of 1X media that do not require frozen storage, so no thawing or mixing of reagents. Simply warm the media and add to PSC culture.
Rapid production of cardiomyocytes The PSC Cardiomyocyte Differentiation Kit is capable of producing contracting cardiomyocytes in as few as 8 days. Differentiated cardiomyocytes can be subsequently maintained in culture for >15 days.
Generation of high quality cardiomyocytes Cardiomyocytes generated using the PSC Cardiomyocyte Differentiation Kit are functionally relevant, expressing key markers such as TNNT2, Nkx2.5, MYH6, and α-actinin; and contract in culture.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeCardiomyocytes
Product TypePSC Cardiomyocyte Differentiation Kit
Quantity1 kit
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
• 500 mL Cardiomyocyte Maintenance Medium • 100 mL Cardiomyocyte Differentiation Medium A • 100 mL Cardiomyocyte Differentiation Medium B
Store at 2–8° and protect from light.
Frequently asked questions (FAQs)
I used the Gibco PSC Cardiomyocyte Differentiation Kit and am not seeing good differentiation with my iPSC line. What should I do?
We recommend always using H9 or H7 ESC line as a control in your experiments. We recommend adjusting the cell density or extending the induction time for difficult-to-differentiate iPSC lines.
I am planning to use the PSC Cardiomyocyte Differentiation Kit. Which markers can I expected my differentiated cells to express?
Cardiomyocytes generated using PSC Cardiomyocyte Differentiation Kit have been tested for key markers such as TNNT2, Nkx2.5, MYH6, and Alpha-Actinin. The Cardiomyocyte Immunocytochemistry Kit (Cat. No. A25973) contains validated antibodies to measure TNNT2 and Nkx2.5 in cultures generated using PSC Cardiomyocyte Differentiation Kit.
How long can I maintain differentiated cells in culture using the Gibco PSC Cardiomyocyte Differentiation Kit?
Differentiated cells can be maintained for a month or longer for long-term studies. We recommend the use of Gibco Geltrex Matrix for long-term cultures.