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Essential 8™ Flex Medium Kit
Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) without theRead more
Catalog number A2858501
Price (USD)
401.00
Each
Price (USD)
401.00
Each
Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) without the need for weekend feeding. Essential 8 Flex provides all of the benefits of Essential 8 Medium: minimized variability of components, reliable and robust cultures with a xeno-free, cGMP produced media; along with the added benefit of flexibility. Maintenance of standard PSCs no longer requires being in the lab 7 days a week.
Flexibility
With Essential 8 Flex Medium, PSCs need only be fed on the day of splitting and the day after. If the cells will be left without feeding for more than 48 hours, such as over a weekend, then simply double the feed volume on Friday.
Reliable and robust cultures
Essential 8 Flex Medium has been shown to support PSC growth for >30 passages without any signs of karyotypic abnormalities and to maintain the ability of iPSCs to differentiate into all three germ line lineages.
Cost effective
Essential 8 Flex Medium is provided in a convenient two-component kit (500 mL basal medium and 10 mL supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human PSCs.
The Essential 8 Flex Medium formulation is based upon that published for Essential 8 Medium by Chen, et al., and allows for control and flexibility of PSC culture medium, without a negative impact to the health of the stem cells.
Flexibility
With Essential 8 Flex Medium, PSCs need only be fed on the day of splitting and the day after. If the cells will be left without feeding for more than 48 hours, such as over a weekend, then simply double the feed volume on Friday.
Reliable and robust cultures
Essential 8 Flex Medium has been shown to support PSC growth for >30 passages without any signs of karyotypic abnormalities and to maintain the ability of iPSCs to differentiate into all three germ line lineages.
Cost effective
Essential 8 Flex Medium is provided in a convenient two-component kit (500 mL basal medium and 10 mL supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human PSCs.
The Essential 8 Flex Medium formulation is based upon that published for Essential 8 Medium by Chen, et al., and allows for control and flexibility of PSC culture medium, without a negative impact to the health of the stem cells.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs), Human Stem Cells
ClassificationXeno-free
Culture TypeFeeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic)
Product TypeStem Cell Media
With AdditivesPhenol Red
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product LineEssential 8™
Quantity1 kit
Shipping ConditionSupplement - dry ice, Basal - ambient
Unit SizeEach
Contents & Storage
• 500 mL Basal Medium (store at 2–8°C and protect from light)
• 10 mL Supplement (store at -5 to -20°C and protect from light)
• 10 mL Supplement (store at -5 to -20°C and protect from light)