The HID Real-Time PCR Software has been fully validated for human identification. The key workflow features include configurable Quantifiler templates as well as instrument calibration, data quality assessment, and integrated sample normalization/STR reaction setup tools for simplified, faster sample processing from quantification to STR analysis. It can automatically calculate the degradation index, male:female mixture ratio, and dilution values, and includes a number of customizable data quality flags for rapid data review. The HID Real-Time PCR Software uses different analysis algorithms than the SDS v1.2.3 software. In our internal comparison testing, the HID Real-Time PCR Software generated CT values with statistically significant differences compared to the SDS v1.2.3 software.