RapidFinder™ Quant Multi-Meat Set

The RapidFinder™ Quant Multi-Meat Set, when used with one or more of the RapidFinder™ Meat ID kits listed below, enables the determination of the percentage of a target meat species with respect to total meat in food and feed samples. Using the included standards, the kit enables quantification of as little as 0.05% target species DNA as compared to other meat DNA in only a few hours.

The RapidFinder Quant Multi-Meat Set is designed to be used in combination with one or more of the following meat species-specific ID kits (available separately):



• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-compatible with any food or feed sample, both raw and processed
• Reliable-Includes multi-meat DNA quantification standard
• Fast-from sample to result in the same day

How It Works
The method relies on quantitative real-time PCR. First, a standard curve is created by serially diluting the included multi-meat DNA standard. Then assays for the target species DNA (from one of the kits listed above) and for all meat DNA (from the RapidFinder™ Quant Multi-Meat Set) are run side-by-side on both the food sample DNA and the DNA standards. This approach enables rapid and accurate quantification and reporting of the percentage of target meat species as compared to total meat in a sample. Included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.

The RapidFinder™ Quant Multi-Meat Set is compatible with Applied Biosystems™ StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ meat species identification and quantification workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.