CytoTune™ EmGFP Sendai Fluorescence Reporter
CytoTune™ EmGFP Sendai Fluorescence Reporter
Invitrogen™

CytoTune™ EmGFP Sendai Fluorescence Reporter

The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS SendaiRead more
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Catalog number A16519
Price (USD)
592.00
Each
Add to cart
Price (USD)
592.00
Each
Add to cart
The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS Sendai reprogramming kits can transduce into a given cell type before you advance to one of these kits. This vector expresses the next-generation Aequorea victoria EGFP gene, encoding the emerald green fluorescent protein (EmGFP). The cell will express the EmGFP, which is bright, photostable, and ideal for testing transduction.

The CytoTune™ EmGFP Sendai Fluorescence Reporter enables a better understanding of how both the original CytoTune™-iPS Sendai Reprogramming kits and the newer CytoTune™-iPS 2.0 Sendai Reprogramming Kit transduces various cell types. The Sendai particle enters a cell via its HN envelope protein that recognizes sialic acid and is widely expressed in mammalian cells, allowing Sendai virus to target a wide range of cell types. The Sendai virus vectors do not enter the nucleus, thus avoiding any DNA phase, as the viral genome remains as RNA in the cytoplasm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSendai Reporter
GeneGFP (EmGFP)
AssayGFP (EmGFP)
Delivery TypeSendai virus
PromoterProprietary
Product LineCytoTune™
Quantity1 Pack
FormLiquid
Unit SizeEach
Contents & Storage
1 Tube, store (at -68°C to -85°C.)

Frequently asked questions (FAQs)

Can I mix the Invitrogen CytoTune-EmGFP Sendai Fluorescence Reporter with the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit?

If you want to use the EmGFP Reporter with reprogramming, it must be added at the time of reprogramming. Cells infected with Sendai virus will most likely be refractive to further infection. Therefore, do not try to add Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit to cells already transduced with Invitrogen CytoTune-EmGFP Sendai Fluorescence Reporter or vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

After addition of Invitrogen CytoTune-EmGFP Sendai Fluorescence Reporter, when can I see expression of EmGFP?

The expression of EmGFP in successfully transduced cells is detectable at 24 hours post-transduction by fluorescence microscopy and reaches maximal levels at 48-72 hours post-transduction.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I use the Invitrogen CytoTune-EmGFP Sendai Fluorescence Reporter?

Add the reporter one time to your cells and monitor for expression. Different cell types may vary in their ability to take up Sendai virus; therefore, we suggest initially transducing your cells with at least 2-3 different MOIs (e.g., 1, 3, and 9). Please refer to the user manual for the full protocol (http://tools.thermofisher.com/content/sfs/manuals/cytotune_emgfp_sendai_reporter_man.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is EmGFP?

Emerald Green Fluorescent Protein (EmGFP) is a form of GFP with brighter green fluorescence, used to report the expression of a gene of interest. EmGFP can be visualized on standard FITC channel.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the Invitrogen CytoTune-EmGFP Sendai Fluorescence Reporter?

The Invitrogen CytoTune-EmGFP Sendai Fluorescence Reporter is a fluorescent control vector carrying the EmGFP gene. The fluorescent control vector allows the determination of whether a cell line of interest is amenable or refractive to infection by Sendai reprogramming vectors.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CytoTune™ EmGFP Sendai Fluorescence Reporter FAQs