CytoTune™-iPS 2.0 Sendai Reprogramming Kit
Get information about CTS CytoTune 2.1, a new reprogramming kit designed for clinical and translational research.
CytoTune™-iPS 2.0 Sendai Reprogramming Kit
Invitrogen™

CytoTune™-iPS 2.0 Sendai Reprogramming Kit

The CytoTune™-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune™-iPS Sendai Reprogramming Kit. Improvements in the 2.0 system includeRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
A165183 Pack
A165171 Pack
Catalog number A16518
Price (USD)
10,080.00
Each
Add to cart
Quantity:
3 Pack
Price (USD)
10,080.00
Each
Add to cart

The CytoTune™-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune™-iPS Sendai Reprogramming Kit. Improvements in the 2.0 system include increased efficiency, lower cytotoxicity, and faster clearance in the generation of induced pluripotent stem cells. This system continues to be easy to use and works with many different somatic cell types, using Sendai particles to deliver Yamanaka factors, which have been shown to be critical for efficient generation of iPSCs. This kit includes three vector preparations: polycistronic Klf4–Oct3/4–Sox2, cMyc, and Klf4.

Features of the CytoTune™-iPS 2.0 Sendai Reprogramming Kit:

• Ease of use—still requires only one application of the virus to obtain successful reprogramming
• Increased efficiency—usually at least 2-fold higher reprogramming efficiencies than the original kit
• Rapid clearance—CytoTune™-iPS products use the non-integrating Sendai virus that remains in the cytoplasm, reducing the likelihood of integration into the host genome. With the CytoTune™-iPS 2.0 Sendai virus, the Sendai backbone and transgenes clear the cytoplasm faster than the original.

The CytoTune™-iPS 2.0 Sendai system continues to be one of the easiest methods available for cell reprogramming. Only one application of the virus is required, instead of the multiple days of transductions required for mRNA-mediated reprogramming. It is also an ideal system for blood cell reprogramming.

The CytoTune™-iPS 2.0 Sendai Reprogramming Kit typically exhibits greater than 2-fold efficiency over the original kit, for both fibroblast and blood reprogramming. Efficiencies greater than 1% have been obtained with both fibroblasts and blood cells. (BJ and CD34+ cells, respectively).

Please refer to each kit's Certificate of Analysis for the appropriate titer and volume recommendations for reprogramming.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Delivery TypeSendai virus
FormatKit
For Use With (Application)Stem Cell Engineering & Reprogramming
Product LineCytoTune™
Product TypeSendai Reprogramming Kit
Quantity3 Pack
PromoterProprietary
Shipping ConditionDry Ice
Sub Cellular LocalizationCytoplasm
Unit SizeEach
Contents & Storage
3 x 3 Tubes, store (at -68°C to -85°C.)

Frequently asked questions (FAQs)

What is the shelf life of the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

The shelf life for the CytoTune-iPS 2.0 Sendai Reprogramming Kit is 2 years when stored at -80 degrees C with no repeated freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the optimal passage number for reprogramming patient fibroblasts using the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

We recommend reprogramming patient cells at the earliest passage possible. However, it is important to have the cells growing and healthy, which can take between 1-4 weeks. The cells are usually ready to reprogram once they have gone through a total of 3-4 passages.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I scale down my CytoTune-iPS 2.0 Sendai reprogramming experiments?

Yes. Initial experiments with fibroblasts have shown that scaling down to a 12-well or 24-well works, but at a potentially reduced efficiency. Cell seeding densities may need to be optimized.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How many cells do I need to start my reprogramming experiment with the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

We recommend seeding approximately 50,000-100,000 fibroblast cells per well of a 6-well to yield 200,000-300,000 cells two days later. This equals a confluency of 50-80% on the day of transduction. Overly confluent cells will result in a decreased transduction efficiency. When cells are overly confluent (>80% confluent), we recommend re-plating the cells to achieve a range of 50-80% confluency on the day of transduction.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between the CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit and the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

The CTS CytoTune-iPS 2.1 Sendai vector formulation does not contain the bovine serum albumin carrier protein. Additionally, the preparation contains no animal-derived components at the primary component level. Each virus (i.e., KOS, L-Myc, and Kfl4) comes in a volume of 200 µL instead of 100 µL. The titer of the CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit can range from 0.5 x 10E8 to 2.0 x 10E8 virus particles per mL whereas the titer of the CytoTune-iPS 2.0 Sendai Reprogramming Kit is typically around 1.0 x 10E8 virus particles per mL.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CytoTune™-iPS 2.0 Sendai Reprogramming Kit FAQs