PSC Neural Induction Medium
After generating neural stem cells (NSCs) using PSC Neural Induction Medium, confirm expression of NSC markers with this convenient multicolor immunocytochemistry kit.
PSC Neural Induction Medium
Gibco™

PSC Neural Induction Medium

Gibco™ PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cellsRead more
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Catalog number A1647801
Price (USD)
700.00
Each
Add to cart
Price (USD)
700.00
Each
Add to cart
Gibco™ PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cells (PSCs) in only 7 days. Unlike existing methodologies, use of PSC Neural Induction Medium does not require the intermediary step of embryoid body (EB) formation, which adds time, labor, and variability. Neural Stem Cells (NSCs) generated using PSC Neural Induction Medium have high expression of NSC markers and can be further differentiated into other neural cell types.

PSC Neural Induction Medium enables:

• Rapid induction of NSCs—high efficiency neural induction in only 7 days, without EB formation
• Scalable production of NSCs:—generate >20 million NSCs starting from 1 million PSCs
• Generation of high quality NSCs—resulting NSCs can be cryopreserved, expanded, and differentiated

Rapid Induction of NSCs
PSC Neural Induction Medium simplifies the NSC generation workflow by eliminating the need to form EBs or rosettes. By removing this step, PSC Neural Induction Medium enables a more reproducible process with high efficiency neural induction in only 7 days.

Scalable Production of NSCs
PSC Neural Induction Medium is a robust medium that has been shown to generate over 20 million NSCs from 1 million PSCs. The medium has been tested with multiple hPSC lines including hiPSCs derived from use of Episomal iPSC Reprogramming Vectors and the CytoTune™-iPS Sendai Reprogramming Kit.

Generation of High Quality NSCs
Neural stem cells generated using PSC Neural Induction Medium have >80% expression of Sox1, Sox2, and Nestin (NSC markers), and <1% expression of Oct4 (pluripotency marker). In addition, expanded NSCs maintained phenotype, exhibited normal karyotype, and could be differentiated into neurons, astrocytes, and oligodendrocytes.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs), Human Stem Cells
Product TypePSC Neural Induction Media
Serum LevelSerum-free
Quantity500 mL
Unit SizeEach
Contents & Storage
• 500 mL Basal medium (store at 2–8°C and protect from light)
• 10 mL Supplement (store at -5 to -20°C and protect from light)

Frequently asked questions (FAQs)

Can PSC Neural Induction Medium be used to differentiate hPSCs into neurospheres or neurons?

PSC Neural Induction Medium is a medium to convert hPSCs into NSCs. At day 7 of neural induction, dissociate P0 NSCs with Accutase. The following recommendations can be use for differentiation of neurons or neurospheres: For differentiation of neurons: Use PSC Neural Induction Medium to convert hPSCs into NSCs. At day 7 of neural induction, dissociate P0 NSCs with Accutase. If dissociated NSCs are plated on a Geltrex-coated culture vessel in neural expansion medium, NSCs will grow as a mono-layer. For NSC expansion, it is necessary to treat NSCs with 5 µM ROCK inhibitor Y27632 at the time of plating to prevent cell death if NSCs are under P4. For differentiation of neurospheres: Use PSC Neural Induction Medium to convert hPSCs into NSCs. At day 7 of neural induction, dissociate P0 NSCs with Accutase. If dissociated NSCs are plated into a non-coated flask in neural expansion medium, dissociated NSCs will re-aggregate into small spheres. NSCs in each sphere will proliferate and form big neurospheres. We have not expanded NSCs in neurosphere format in-house. You can test expanding NSCs in neurospheres with and without ROCK inhibitor Y27632 to determine which is optimal for your workflow. Neural stem cells in adherent or neurosphere conditions can be differentiated into neurons using the appropriate protocol.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Have you performed flow cytometry or qPCR studies to determine the expression of NKX2.1 in NSCs generated from iPSC using PSC Neural Induction Medium?

Studies have not been performed using flow cytometry or qPCR to detect NKX2.1 expression in NSCs induced by Neural Induction Medium.

Is it normal to see loss of pluripotency markers (i.e. SSEA-4, Oct-4, and Tra1-60) and still test positive for SOX2 expression when NSCs are cultured in neural expansion medium?

Yes, this is normal. SOX2 is expressed by both hPSCs and hPSC-derived NSCs, so you will see its expression in the induced NSCs from iPSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can NSCs induced by PSC Neural Induction Medium be differentiated into neurons on glass slides/coverslips coated with laminin and poly-L-ornithine?

NSCs induced using the PSC Neural Induction Medium can be differentiated on glass slides/coverslips, but the glass should be cleaned well before coating. We recommend the following cleaning protocol:
1. Treat glass cover slips with1M HCl at RT for 2-3 h on a shaker.
2. Rinse with tap water 5 times, and then double distilled water for 2 times
3. Store cleaned cover slips in 70% ethanol.
4. Dry cover slips by transferring cover slips into culture plate with sterile forceps before poly-L-ornithine and laminin coating.

Can I maintain differentiated neurospheres in Neurobasal+B27+N2+bFGF+EGF?

Neurospheres can be plated on laminin coated culture plates for neuron differentiation. The issue is that it is difficult to control the plating density of neurospheres. Alternatively, neurospheres can be dissociated into single cells and plate single cell suspension at a certain density such as 1-5 x 10^4 cells/cm2 onto laminin coated plates for neuron differentiation. For general neuron differentiation, Neurobasal+B27+N2 can be used. Growth factors such as BDNF and/or GDNF can be added into medium for improving survival of differentiating NSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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