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Expi293™ Expression System Kit
The Expi293 Expression System is a complete solution for rapid, high-yield protein production from mammalian 293 cells. It is basedRead more
Catalog number A14635
Price (USD)
2,990.00
Each
Price (USD)
2,990.00
Each
The Expi293 Expression System is a complete solution for rapid, high-yield protein production from mammalian 293 cells. It is based on integrated components including high-density Expi293F cells in Expi293 Expression Medium and the cationic lipid-based ExpiFectamine 293 transfection reagent in combination with specialized transfection enhancers.
The Expi293 Expression System features:
• Up to 1 g/L of protein; 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle 293 Expression System
• Rapid production of recombinant proteins in 5–7 days
• High density cultures of Expi293F cells that result in more expressing cells per milliliter of culture
• Native folding and mammalian post-translational modifications of expressed proteins
• Easy, robust culture and transfection protocols
• Scalable expression from <1 mL to >10 L
The components included in the Expi293 Expression System Kit are: two vials of frozen Expi293F cells, one liter of Expi293 Expression Medium, one ExpiFectamine 293 Transfection kit sufficient to transfect 1 liter of culture, Opti-MEM I Reduced Serum Medium, and an antibody-expressing positive control vector. All components of the Expi293 Expression System are also available for individual purchase.
Engineered Expi293F cell lines for a range of applications are also available for individual purchase or as part of kits: Expi293F GnTI- Cells (Cat. No. A39240). Expi293F Inducible Cells (Cat. No. A39241), and Expi293F Inducible GnTI- Cells (Cat. No. A39242).
The Expi293 Expression System features:
• Up to 1 g/L of protein; 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle 293 Expression System
• Rapid production of recombinant proteins in 5–7 days
• High density cultures of Expi293F cells that result in more expressing cells per milliliter of culture
• Native folding and mammalian post-translational modifications of expressed proteins
• Easy, robust culture and transfection protocols
• Scalable expression from <1 mL to >10 L
The components included in the Expi293 Expression System Kit are: two vials of frozen Expi293F cells, one liter of Expi293 Expression Medium, one ExpiFectamine 293 Transfection kit sufficient to transfect 1 liter of culture, Opti-MEM I Reduced Serum Medium, and an antibody-expressing positive control vector. All components of the Expi293 Expression System are also available for individual purchase.
Engineered Expi293F cell lines for a range of applications are also available for individual purchase or as part of kits: Expi293F GnTI- Cells (Cat. No. A39240). Expi293F Inducible Cells (Cat. No. A39241), and Expi293F Inducible GnTI- Cells (Cat. No. A39242).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineExpi293F™
Delivery TypeTransfection
Cell Type293-derived Cells
PromoterCMV
Transfection TechniqueLipid-based Transfection
Expression SystemMammalian
Expression StabilityTransient
For Use With (Application)Protein Expression
Key FunctionsProtein Production, Constitutive Expression
No. of Reactions33
Product LineExpi293™
Product TypeExpression System Kit
Protein TagUntagged
Quantity1 kit
System TypeExpi293™ Expression System
Unit SizeEach
Contents & Storage
2 vials frozen Expi293F cells
1 bottle 1000 mL Expi293 Expression Medium
1 ExpiFectamine 293 transfection kit for 1 L of culture
1 vial Antibody Expressing Positive Control Vector
1 bottle 100 mL Opti-MEM Reduced Serum Medium
Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen
1 bottle 1000 mL Expi293 Expression Medium
1 ExpiFectamine 293 transfection kit for 1 L of culture
1 vial Antibody Expressing Positive Control Vector
1 bottle 100 mL Opti-MEM Reduced Serum Medium
Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen