BG-11 Media, optimized for cyanobacteria
BG-11 Media, optimized for cyanobacteria
Gibco™

BG-11 Media, optimized for cyanobacteria

Gibco™ BG-11 Media is optimized for the growth and maintenance of select Cyanobacteria including Synechococcus elongates. The formulation comes ready-to-useRead more
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Catalog NumberQuantity
A13799011 L
A1379902
Catalog number A1379901
Price (USD)
61.75
Each
Add to cart
Quantity:
1 L
Price (USD)
61.75
Each
Add to cart
Gibco™ BG-11 Media is optimized for the growth and maintenance of select Cyanobacteria including Synechococcus elongates. The formulation comes ready-to-use at 1X to let you avoid laborious media preparation steps.

Each Gibco™ BG-11 Media comes in the award winning Gibco™ bottle designed for easier use in the biosafety cabinet, minimizing the risk of contamination, and helps you perform cell culture more consistently. Together, the superior packaging and quality, greater reliability, and improved consistency in Cyanobacteria culture, results in better overall efficiency and more robust data (Fig. 1, 2).

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Specifications
FormLiquid
Media TypeAlgal Media
Preparation MethodNone (Ready-to-Use)
Product TypeMicrobiology Media
Target Organism ClassCyanobacteria
Green FeaturesSustainable packaging
Quantity1 L
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Gibco™ BG-11 Growth Media contains:

•  1 L Gibco™ BG-11 Media — Store at 4 °C

Frequently asked questions (FAQs)

I'm getting low basal expression of my gene of interest when using the pSyn_1 vector. What do you suggest I try?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter for pSyn_6, on the other hand, is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving the high level expression of your gene of interest.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the difference between pSyn_1 and pSyn_6?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter was taken from the solanesyl diphosphate synthase gene from Synechocystis sp. Strain PCC 6803. Note: Use of this weak constitutive promoter is a good fit for applications that are hindered by strong expression, such as pathway engineering or complementation of mutant genes normally expressed at low levels (Simkovsky et al., 2012).

The promoter for pSyn_6 is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving high-level expression of your gene of interest. The pSyn-6 vector also includes a ribosome-binding site (GAAGGAG) for efficient initiation of translation, as well as a stop codon. A 6xHis TEV and a V5-His epitope tag is also included in the vector backbone for detection/purification of the gene of interest. NS1 (neutral site 1) homologous recombination sites are present in both vectors for the integration of the vector into the Synechococcus elongatus genome.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the shelf-life/competency of Synechococcus?

After 6 months of storage, the Synechococcus did not lose their competency. We have not yet tested longer storage points.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the mechanism of integration when using the Synechococcus expression kits?

A double homologous recombination event occurs between the Neutral Site 1 in the genome and between NSa and NSb in the plasmid. This results in the gene of interest and spectinomycin resistance integrating into the genome within the Neutral Site 1.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the strain of Synechococcus used in your kits?

We no longer offer any strains. Our reagents and protocols were developed using Synechococcus elongatus strain PCC 7942, a model cyanobacterium.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all BG-11 Media, optimized for cyanobacteria FAQs