CTS™ Synth-a-Freeze™ Medium
CTS™ Synth-a-Freeze™ Medium
Gibco™

CTS™ Synth-a-Freeze™ Medium

CTS™ (Cell Therapy Systems) Synth-a-Freeze™ medium is a chemically defined, protein-free liquid cryopreservation medium intended for freezing and storing aRead more
Have Questions?
Catalog number A1371301
Price (USD)
227.00
Each
Add to cart
Price (USD)
227.00
Each
Add to cart

CTS™ (Cell Therapy Systems) Synth-a-Freeze™ medium is a chemically defined, protein-free liquid cryopreservation medium intended for freezing and storing a variety of mammalian cell types including stem cells. Synth-a-Freeze™ CTS™ media is optimally formulated without antibiotics, antimycotics, hormones, growth factors, serum, or protein and buffered with HEPES and sodium bicarbonate.

• Use for cryopreserving a variety of primary and stem cells
• Save time and effort with 4 degree stable storage of thawed media and compatibility with a variety freezing protocols
• Be ready for clinical research with this cGmP manufactured, chemically defined, protein-free formula

Cryopreserve a Variety of Cell Types
Cell Therapy Systems Synth-a-Freeze™ CTS™ media performs comparable to our standard, serum-containing cryopreservation medium for cryopreserving a variety of cell types including human Embryonic Stem Cells, Neural Stem Cells (Ref 1), Mesenchymal Stem Cells and Keratinocytes (Ref 2). We do not recommend using Synth-a-Freeze™ media for cryopreservation of human epidermal melanocytes.

Simple Incorporation into Your Current Freezing Protocol
Synth-a-Freeze™ CTS™ media can be used with any standard freezing protocol. Just add thawed Cell Therapy Systems Synth-a-Freeze™ CTS™ media to detached and pelleted cells, aliquot, and freeze. For optimal results, the use of a controlled-rate freezer is recommended.

Simplify Your Transition into Clinical Research
The Gibco™ Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco™ Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products.

CAUTION: Not intended for direct administration into humans or animals

References:
• S. Shin and M. Vemuri. Culture and Differentiation of Human Neural Stem Cells. L.C. Doering (ed). Protocols for Neural Cell Culture. Springer Protocol Handbooks.
• S. Lemaire, K. Kosowska-Shick, P. Appelbaum, G. Verween, P. Tulkens, and F. Van Bambeke. Cellular Pharmacodynamics of the Novel Biaryloxazolidinone Radezolid: Studies with Infected Phagocytic and Nonphagocytic cells, Using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila. Antimicrob Agents Chemother 54(6) 2010: 2549-2559.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeVarious (including types of human Embryonic Stem Cells, Neural Stem Cells, Mesenchymal Stem Cells, and Keratinocytes)
ClassificationAnimal Origin-free
FormLiquid
Product TypeCryopreservation Medium
Shelf Life18 months from date of manufacture
SterilitySterile-filtered
With AdditivesDMSO (10%)
FormatBottle
For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing
Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820
Quantity50 mL
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Store at –20°C.

Frequently asked questions (FAQs)

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

If the Synth-a-Freeze Cryopreservation Medium is not suitable for melanocytes, how should I freeze melanocytes?

We recommend using DMEM plus 10% FBS and 10% DMSO, or the Recovery Cell Culture Freezing Medium. You can try the same general recommendations for freezing cells, just changing the freezing solution.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there a Drug Master File for the Synth-a-Freeze Cryopreservation Medium?

There isn't a Drug Master File, but there is a Device Master File on file with the FDA. Please contact our Licensing team at outlicensing@thermofisher.com in order to obtain instructions for referencing this Device Master File.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can I use Synth-a-Freeze Cryopreservation Medium for?

Synth-a-Freeze Cryopreservation Medium can be used with any standard freezing protocol. It offers performance comparable to that of our standard, serum-containing cryopreservation medium for cyropreserving a variety of cell types including human keratinocytes, embryonic stem cells, neural stem cells, and mesenchymal stem cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Was the tissue cryopreserved prior to isolation of primary cells?

No, the tissue was not cryopreserved.